- CITATIONS: 1
|Application ||WB, E|
|Other Accession||Q3TJ91, Q8K4K5, Q80Y17, Q15334, Q8MKF0|
|Predicted||Mouse, Rat, Bovine|
|Calculated MW||113448 Da|
|Other Names||Lethal(2) giant larvae protein homolog 2, HGL, LLGL2|
|Target/Specificity||This LLGL1/2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S645/S649 of human LLGL1/2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Bi-Phospho-LLGL1/2(S653/S649) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Part of a complex with GPSM2/LGN, PRKCI/aPKC and PARD6B/Par-6, which may ensure the correct organization and orientation of bipolar spindles for normal cell division. This complex plays roles in the initial phase of the establishment of epithelial cell polarity.|
|Cellular Location||Cytoplasm. Note=Localized in the perinuclear structure and faintly at the cell-cell contacts sites in the interphase. Localized at the cell periphery during metaphase. Cortical localization in mitotic cells. Found in the lateral region of polarized epithelial cells|
Provided below are standard protocols that you may find useful for product applications.
LLGL1 is a protein that is similar to a tumor suppressor in Drosophila. The protein is part of a cytoskeletal network and is associated with nonmuscle myosin II heavy chain and a kinase that specifically phosphorylates this protein at serine residues. The gene for LLGL1 is located within the Smith-Magenis syndrome region on chromosome 17. LLGL2 is a protein similar to lethal (2) giant larvae of Drosophila. In fly, the protein's ability to localize cell fate determinants is regulated by the atypical protein kinase C (aPKC). In human, this protein interacts with aPKC-containing complexes and is cortically localized in mitotic cells.
Schimanski, C.C., et al., Oncogene 24(19):3100-3109 (2005). Grifoni, D., et al., Oncogene 23(53):8688-8694 (2004). Katoh, M., et al., Int. J. Oncol. 24(3):737-742 (2004). Bi, W., et al., Genome Res. 12(5):713-728 (2002). Ludford-Menting, M.J., et al., J. Biol. Chem. 277(6):4477-4484 (2002). Yasumi, M., et al., J. Biol. Chem. 280(8):6761-6765 (2005).
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