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CCT8L2 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - CCT8L2 Antibody (N-Term) AP22024a
    All lanes : Anti-CCT8L2 Antibody (N-Term) at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lane 3: A673 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • FC - CCT8L2 Antibody (N-Term) AP22024a
    Overlay histogram showing Jurkat cells stained with AP22024a (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22024a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q96SF2
Other Accession A6NM43
Reactivity Human
Predicted Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 59388 Da
Additional Information
Gene ID 150160
Other Names Putative T-complex protein 1 subunit theta-like 2, CCT8L2, CESK1
Target/Specificity This CCT8L2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 92-124 amino acids from human CCT8L2.
Dilution WB~~1:2000
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCCT8L2 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCT8L2
Synonyms CESK1
Function Possible molecular chaperone; assists the folding of proteins upon ATP hydrolysis.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:P40227}.
Research Areas
Citations (0)
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Background

Possible molecular chaperone; assists the folding of proteins upon ATP hydrolysis.

References

Riazi M.A.,et al.Genomics 62:90-94(1999).
Collins J.E.,et al.Genome Biol. 5:R84.1-R84.11(2004).
Dunham I.,et al.Nature 402:489-495(1999).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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$ 365.00
$ 140.00
Cat# AP22024a
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