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RPL4 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

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  • FC - RPL4 Antibody (N-Term) AP22030a
    Overlay histogram showing U-2OS cells stained with AP22030a (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22030a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - RPL4 Antibody (N-Term) AP22030a
    All lanes : Anti-RPL4 Antibody (N-Term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Raji whole cell lysate Lane 5: NCI-H929 whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P36578
Other Accession Q58DW0, Q28346, Q9D8E6, Q5RCR3
Reactivity Human
Predicted Bovine, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 47697 Da
Additional Information
Gene ID 6124
Other Names 60S ribosomal protein L4, 60S ribosomal protein L1, RPL4, RPL1
Target/Specificity This RPL4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 119-149 amino acids from human RPL4.
Dilution FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPL4 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPL4
Synonyms RPL1
Citations (0)

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Bagni C.,et al.Biochim. Biophys. Acta 1216:475-478(1993).
Bagni C.,et al.Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
Kato S.,et al.Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases.
Yoshihama M.,et al.Genome Res. 12:379-390(2002).
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 295.00
$ 99.00
Cat# AP22030a
(40 western blots)
Availability: In Stock
Bulk Size
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