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Cyclophilin B Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • FC - Cyclophilin B Antibody AP22114a
    Overlay histogram showing Hela cells stained with AP22114a (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22114a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - Cyclophilin B Antibody AP22114a
    All lanes : Anti-Cyclophilin B Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: BA/F3 whole cell lysate Lane 5: NIH/3T3 whole cell lysate lane 6: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Cyclophilin B Antibody AP22114a
    All lanes : Anti-Cyclophilin B Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: BA/F3 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession P23284
Other Accession P24369
Reactivity Human, Mouse, Rat
Predicted Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 23743 Da
Additional Information
Gene ID 5479
Other Names Peptidyl-prolyl cis-trans isomerase B, PPIase B, 5.2.1.8, CYP-S1, Cyclophilin B, Rotamase B, S-cyclophilin, SCYLP, PPIB, CYPB
Target/Specificity This Cyclophilin B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 161-195 amino acids from the human region of human Cyclophilin B.
Dilution FC~~1:25
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCyclophilin B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPIB
Synonyms CYPB
Function PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Cellular Location Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Research Areas
Citations (0)

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Background

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

References

Spik G.,et al.J. Biol. Chem. 266:10735-10738(1991).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Zody M.C.,et al.Nature 440:671-675(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
$ 99.00
Cat# AP22114a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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