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TIGIT Antibody

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - TIGIT Antibody AP22132a
    All lanes : Anti-TIGIT Antibody at 1:2000 dilution Lane 1: EL4 whole cell lysate Lane 2: human fetal thymus lysate Lane 3: human spleen lysate Lane 4: HUT78 whole cell lysate Lane 5: Jurkat whole cell lysate Lane 6: MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q495A1
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 26319 Da
Additional Information
Gene ID 201633
Other Names T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain-containing protein 3, TIGIT, VSIG9, VSTM3
Target/Specificity This TIGIT antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 12-142 amino acids from human TIGIT.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTIGIT Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms VSIG9, VSTM3
Function Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location Expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level)
Research Areas
Citations (0)

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Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.


Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Yu X.,et al.Nat. Immunol. 10:48-57(2009).
Stengel K.F.,et al.Proc. Natl. Acad. Sci. U.S.A. 109:5399-5404(2012).

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$ 295.00
$ 99.00
Cat# AP22132a
(40 western blots)
Availability: In Stock
Bulk Size
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