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SLC2A8 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • FC - SLC2A8 Antibody (Center) AP22138c
    Overlay histogram showing U-2 OS cells stained with AP22138c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22138c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • IHC-P - SLC2A8 Antibody (Center) AP22138c
    AP22138c staining SLC2A8 in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - SLC2A8 Antibody (Center) AP22138c
    All lanes : Anti-SLC2A8 Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: 293 whole cell lysate Lane 3: human testis lysate Lane 4: Caco2 whole cell lysate Lane 5: mouse testis lysate Lane 6: rat testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 51 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q9NY64
Other Accession P58354
Reactivity Human, Mouse, Rat
Predicted Bovine
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 50819 Da
Additional Information
Gene ID 29988
Other Names Solute carrier family 2, facilitated glucose transporter member 8, Glucose transporter type 8, GLUT-8, Glucose transporter type X1, SLC2A8, GLUT8, GLUTX1
Target/Specificity This SLC2A8 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 260-292 amino acids from the Central region of human SLC2A8.
Dilution FC~~1:25
IHC-P~~1:25
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSLC2A8 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SLC2A8
Synonyms GLUT8, GLUTX1
Function Insulin-regulated facilitative glucose transporter. Binds cytochalasin B in a glucose-inhibitable manner. Seems to be a dual-specific sugar transporter as it is inhibitable by fructose (By similarity).
Cellular Location Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein Note=Principally intracellular. May move between intracellular vesicles and the plasma membrane. The dileucine internalization motif is critical for intracellular sequestration (By similarity)
Tissue Location Highly expressed in testis, but not in testicular carcinoma. Lower amounts present in most other tissues
Research Areas
Citations (0)

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Background

Insulin-regulated facilitative glucose transporter. Binds cytochalasin B in a glucose-inhibitable manner. Seems to be a dual-specific sugar transporter as it is inhibitable by fructose (By similarity).

References

Doege H.,et al.J. Biol. Chem. 275:16275-16280(2000).
Ibberson M.R.,et al.J. Biol. Chem. 275:4607-4612(2000).
Humphray S.J.,et al.Nature 429:369-374(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).

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$ 295.00
$ 99.00
Cat# AP22138c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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