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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PCSK1 Antibody (C-Term)   

PCSK1 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • FC - PCSK1 Antibody (C-Term) AP22139b
    Overlay histogram showing A549 cells stained with AP22139b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22139b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - PCSK1 Antibody (C-Term) AP22139b
    All lanes : Anti-PCSK1 Antibody (C-Term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: A549 whole cell lysate Lane 3: PC-12 whole cell lysate Lane 4: mouse brain lysate Lane 5: NCI-H460 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession P29120
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 84152 Da
Additional Information
Gene ID 5122
Other Names Neuroendocrine convertase 1, NEC 1, 3.4.21.93, Prohormone convertase 1, Proprotein convertase 1, PC1, PCSK1, NEC1
Target/Specificity This PCSK1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 682-715 amino acids from human PCSK1.
Dilution FC~~1:25
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPCSK1 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PCSK1
Synonyms NEC1
Function Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP.
Cellular Location Cytoplasmic vesicle, secretory vesicle. Note=Localized in the secretion granules
Research Areas
Citations (0)

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Background

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin.

References

Creemers J.W.M.,et al.FEBS Lett. 300:82-88(1992).
Seidah N.G.,et al.DNA Cell Biol. 11:283-289(1992).
Ohagi S.,et al.Diabetes 45:897-901(1996).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Schmutz J.,et al.Nature 431:268-274(2004).

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$ 295.00
$ 99.00
Cat# AP22139b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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