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DBN1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - DBN1 Antibody (Center) AP22154c
    All lanes : Anti-DBN1 Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lane 4: mouse brain lysate Lane 5: Neuro-2a whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 71 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q16643
Other Accession Q9QXS6, Q07266
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Additional Information
Other Names Drebrin, Developmentally-regulated brain protein, DBN1, D0S117E
Target/Specificity This DBN1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 194-228 amino acids from the Central region of human DBN1.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDBN1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DBN1
Synonyms D0S117E
Function Drebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites. Required for actin polymerization at immunological synapses (IS) and for CXCR4 recruitment to IS.
Cellular Location Cytoplasm. Cytoplasm, cell cortex. Cell junction. Cell projection {ECO:0000250|UniProtKB:Q9QXS6}. Cell projection, growth cone {ECO:0000250|UniProtKB:Q9QXS6}. Note=In the absence of antigen, evenly distributed throughout subcortical regions of the T-cell membrane and cytoplasm. In the presence of antigen, distributes to the immunological synapse forming at the T-cell-APC contact area, where it localizes at the peripheral and distal supramolecular activation clusters (SMAC) (PubMed:20215400). Colocalized with DBN1, RUFY3 and F-actin at the transitional domain of the axonal growth cone (By similarity). {ECO:0000250|UniProtKB:Q9QXS6, ECO:0000269|PubMed:20215400}
Tissue Location Brain neurons. Also found in the heart, placenta, skeletal muscle, kidney and pancreas. Expressed in peripheral blood lymphocytes, including T-cells (at protein level). EMBL; D17530; BAA04480.1; -; mRNA EMBL; U00802; AAA16256.1; -; mRNA EMBL; AK314645; BAG37207.1; -; mRNA EMBL; AL110225; CAB53683.1; -; mRNA EMBL; AC145098; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; BC000283; AAH00283.1; -; mRNA EMBL; BC007281; AAH07281.1; -; mRNA EMBL; BC007567; AAH07567.1; -; mRNA EMBL; BC114553; -; NOT_ANNOTATED_CDS; mRNA CCDS; CCDS4420.1; -. [Q16643-1] CCDS; CCDS4421.1; -. [Q16643-2] PIR; JN0809; JN0809 PIR; T14763; T14763 RefSeq; NP_004386.2; NM_004395.3 RefSeq; NP_543157.1; NM_080881.2 RefSeq; XP_005265884.1; XM_005265827.3. [Q16643-3] RefSeq; XP_016864629.1; XM_017009140.1. [Q16643-2] UniGene; Hs.130316; - PDB; 5Y1Z; X-ray; 2.68 A; A/B=1-135 PDBsum; 5Y1Z; - ProteinModelPortal; Q16643; - SMR; Q16643; - BioGrid; 107995; 239 IntAct; Q16643; 224 MINT; Q16643; - STRING; 9606.ENSP00000292385; - iPTMnet; Q16643; - PhosphoSitePlus; Q16643; - SwissPalm; Q16643; - BioMuta; DBN1; - DMDM; 215274247; - OGP; Q16643; - EPD; Q16643; - MaxQB; Q16643; - PaxDb; Q16643; - PeptideAtlas; Q16643; - PRIDE; Q16643; - ProteomicsDB; 60996; - ProteomicsDB; 60997; -. [Q16643-2] DNASU; 1627; - Ensembl; ENST00000292385; ENSP00000292385; ENSG00000113758. [Q16643-2] Ensembl; ENST00000309007; ENSP00000308532; ENSG00000113758. [Q16643-1] Ensembl; ENST00000393565; ENSP00000377195; ENSG00000113758. [Q16643-3] GeneID; 1627; - KEGG; hsa:1627; - UCSC; uc003mgx.3; human. [Q16643-1] CTD; 1627; - DisGeNET; 1627; - EuPathDB; HostDB:ENSG00000113758.13; - GeneCards; DBN1; - H-InvDB; HIX0005464; - HGNC; HGNC:2695; DBN1 HPA; HPA051452; - HPA; HPA056940; - MIM; 126660; gene neXtProt; NX_Q16643; - OpenTargets; ENSG00000113758; - PharmGKB; PA27163; - eggNOG; ENOG410IP00; Eukaryota eggNOG; ENOG410YAV7; LUCA GeneTree; ENSGT00530000062953; - HOGENOM; HOG000015304; - HOVERGEN; HBG000823; - InParanoid; Q16643; - OMA; PCSHLDS; - OrthoDB; EOG091G05KP; - PhylomeDB; Q16643; - TreeFam; TF318935; - SignaLink; Q16643; - ChiTaRS; DBN1; human GeneWiki; DBN1; - GenomeRNAi; 1627; - PRO; PR:Q16643; - Proteomes; UP000005640; Chromosome 5 Bgee; ENSG00000113758; - CleanEx; HS_DBN1; - ExpressionAtlas; Q16643; baseline and differential Genevisible; Q16643; HS GO; GO:0015629; C:actin cytoskeleton; ISS:UniProtKB GO; GO:0042641; C:actomyosin; NAS:UniProtKB GO; GO:0030863; C:cortical cytoskeleton; TAS:ARUK-UCL GO; GO:0005737; C:cytoplasm; NAS:UniProtKB GO; GO:0005856; C:cytoskeleton; IDA:ARUK-UCL GO; GO:0030425; C:dendrite; NAS:UniProtKB GO; GO:0005921; C:gap junction; IEA:Ensembl GO; GO:0030426; C:growth cone; ISS:UniProtKB GO; GO:0005886; C:plasma membrane; IEA:Ensembl GO; GO:0003779; F:actin binding; TAS:ProtInc GO; GO:0045296; F:cadherin binding; HDA:BHF-UCL GO; GO:0005522; F:profilin binding; ISS:UniProtKB GO; GO:0007015; P:actin filament organization; ISS:UniProtKB GO; GO:0010643; P:cell communication by chemical coupling; IEA:Ensembl GO; GO:0010644; P:cell communication by electrical coupling; IEA:Ensembl GO; GO:0032507; P:maintenance of protein location in cell; IEA:Ensembl GO; GO:0061351; P:neural precursor cell proliferation; IEA:Ensembl GO; GO:0050773; P:regulation of dendrite development; NAS:UniProtKB GO; GO:0048168; P:regulation of neuronal synaptic plasticity; NAS:UniProtKB Gene3D; 3.40.20.10; -; 1 InterPro; IPR002108; ADF-H InterPro; IPR029006; ADF-H/Gelsolin-like_dom_sf InterPro; IPR028438; Drebrin PANTHER; PTHR10829:SF1; PTHR10829:SF1; 1 Pfam; PF00241; Cofilin_ADF; 1 SMART; SM00102; ADF; 1 PROSITE; PS51263; ADF_H; 1 1: Evidence at protein level; 3D-structure; Acetylation; Actin-binding; Alternative splicing; Cell junction; Cell projection; Complete proteome; Cytoplasm; Developmental protein; Differentiation; Direct protein sequencing; Neurogenesis; Phosphoprotein; Polymorphism; Reference proteome INIT_MET 1 1 Removed. {ECO:0000244|PubMed:19413330, ECO:0000269|PubMed:12665801, ECO:0000269|Ref.8} CHAIN 2 649 Drebrin /FTId=PRO_0000080008 DOMAIN 3 134 ADF-H. {ECO:0000255|PROSITE- ProRule:PRU00599} MOD_RES 2 2 N-acetylalanine {ECO:0000244|PubMed:19413330, ECO:0000269|PubMed:12665801, ECO:0000269|Ref.8} MOD_RES 141 141 Phosphoserine MOD_RES 142 142 Phosphoserine {ECO:0000244|PubMed:18669648, ECO:0000244|PubMed:20068231, ECO:0000244|PubMed:21406692, ECO:0000244|PubMed:23186163, ECO:0000269|Ref.8} MOD_RES 331 331 Phosphothreonine MOD_RES 335 335 Phosphothreonine MOD_RES 337 337 Phosphoserine MOD_RES 339 339 Phosphoserine MOD_RES 345 345 Phosphoserine {ECO:0000250|UniProtKB:Q9QXS6} MOD_RES 346 346 Phosphothreonine MOD_RES 416 416 Phosphoserine MOD_RES 497 497 Phosphothreonine MOD_RES 601 601 Phosphoserine {ECO:0000250|UniProtKB:Q9QXS6} VAR_SEQ 4 29 VSFSGHRLELLAAYEEVIREESAADW -> HPWHGTAALAS SQAWRDGRERQALVSCR (in isoform 2) /FTId=VSP_028175 VAR_SEQ 319 319 G -> GRPYCPFIKASDSGPSSSSSSSSSPPRTPFPYITCH RTPNLSSSLPC (in isoform 3) /FTId=VSP_053443 VARIANT 278 278 E -> K (in a breast cancer sample; somatic mutation) /FTId=VAR_035910 VARIANT 446 446 I -> V (in dbSNP:rs2544809) {ECO:0000269|PubMed:15489334, ECO:0000269|PubMed:17974005, ECO:0000269|PubMed:8216329, ECO:0000269|Ref.2} /FTId=VAR_047365 VARIANT 553 553 S -> P (in dbSNP:rs28538572) {ECO:0000269|PubMed:15489334, ECO:0000269|PubMed:17974005, ECO:0000269|PubMed:8216329, ECO:0000269|Ref.2} /FTId=VAR_047366 VARIANT 640 640 E -> Q (in a breast cancer sample; somatic mutation) /FTId=VAR_035911 HELIX 10 21 {ECO:0000244|PDB:5Y1Z} STRAND 23 26 {ECO:0000244|PDB:5Y1Z} STRAND 29 34 {ECO:0000244|PDB:5Y1Z} STRAND 40 49 {ECO:0000244|PDB:5Y1Z} HELIX 50 54 {ECO:0000244|PDB:5Y1Z} HELIX 55 57 {ECO:0000244|PDB:5Y1Z} STRAND 62 70 {ECO:0000244|PDB:5Y1Z} STRAND 72 74 {ECO:0000244|PDB:5Y1Z} STRAND 79 86 {ECO:0000244|PDB:5Y1Z} HELIX 92 99 {ECO:0000244|PDB:5Y1Z} HELIX 102 108 {ECO:0000244|PDB:5Y1Z} STRAND 114 120 {ECO:0000244|PDB:5Y1Z} HELIX 121 123 {ECO:0000244|PDB:5Y1Z} HELIX 126 132 {ECO:0000244|PDB:5Y1Z} SEQUENCE 649 AA; 71429 MW; A7DF1AE3776C0BEA CRC64; MAGVSFSGHR LELLAAYEEV IREESAADWA LYTYEDGSDD LKLAASGEGG LQELSGHFEN QKVMYGFCSV KDSQAALPKY VLINWVGEDV PDARKCACAS HVAKVAEFFQ GVDVIVNASS VEDIDAGAIG QRLSNGLARL SSPVLHRLRL REDENAEPVG TTYQKTDAAV EMKRINREQF WEQAKKEEEL RKEEERKKAL DERLRFEQER MEQERQEQEE RERRYREREQ QIEEHRRKQQ TLEAEEAKRR LKEQSIFGDH RDEEEETHMK KSESEVEEAA AIIAQRPDNP REFFKQQERV ASASAGSCDV PSPFNHRPGS HLDSHRRMAP TPIPTRSPSD SSTASTPVAE QIERALDEVT SSQPPPLPPP PPPAQETQEP SPILDSEETR AAAPQAWAGP MEEPPQAQAP PRGPGSPAED LMFMESAEQA VLAAPVEPAT ADATEIHDAA DTIETDTATA DTTVANNVPP AATSLIDLWP GNGEGASTLQ GEPRAPTPPS GTEVTLAEVP LLDEVAPEPL LPAGEGCATL LNFDELPEPP ATFCDPEEVE GESLAAPQTP TLPSALEELE QEQEPEPHLL TNGETTQKEG TQASEGYFSQ SQEEEFAQSE ELCAKAPPPV FYNKPPEIDI TCWDADPVPE EEEGFEGGD
Research Areas
Citations (0)

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Background

Drebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites. Required for actin polymerization at immunological synapses (IS) and for CXCR4 recruitment to IS.

References

Toda M.,et al.Biochem. Biophys. Res. Commun. 196:468-472(1993).
Fisher L.W.,et al.Neurosci. Res. Commun. 14:35-42(1994).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Schmutz J.,et al.Nature 431:268-274(2004).

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$ 295.00
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Cat# AP22154c
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