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SLC2A3 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • FC - SLC2A3 Antibody (C-Term) AP22174b
    Overlay histogram showing U-2 OS cells stained with AP22174b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22174b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - SLC2A3 Antibody (C-Term) AP22174b
    All lanes : Anti-SLC2A3 Antibody (C-Term) at 1:2000 dilution Lane 1: C6 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lane 3: human brain lysate Lane 4: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession P11169
Other Accession Q8TDB8, Q5R608, Q9XSC2
Reactivity Human, Mouse, Rat
Predicted Rabbit
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 53924 Da
Additional Information
Gene ID 6515
Other Names Solute carrier family 2, facilitated glucose transporter member 3, Glucose transporter type 3, brain, GLUT-3, SLC2A3, GLUT3
Target/Specificity This SLC2A3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 432-463 amino acids from human SLC2A3.
Dilution FC~~1:25
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSLC2A3 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SLC2A3
Synonyms GLUT3
Function Facilitative glucose transporter that can also mediate the uptake of various other monosaccharides across the cell membrane (PubMed:9477959, PubMed:26176916). Mediates the uptake of glucose, 2-deoxyglucose, galactose, mannose, xylose and fucose, and probably also dehydroascorbate (PubMed:9477959, PubMed:26176916). Does not mediate fructose transport (PubMed:9477959, PubMed:26176916).
Cellular Location Cell membrane; Multi-pass membrane protein
Tissue Location Highly expressed in brain. Expressed in many tissues.
Research Areas
Citations (0)

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Background

Facilitative glucose transporter. Probably a neuronal glucose transporter.

References

Kayano T.,et al.J. Biol. Chem. 263:15245-15248(1988).
Stuart C.A.,et al.Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
$ 99.00
Cat# AP22174b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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