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TK1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • FC - TK1 Antibody (Center) AP22210c
    Overlay histogram showing U-2 OS cells stained with AP22210c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22210c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • IHC-P - TK1 Antibody (Center) AP22210c
    AP22210c staining TK1 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - TK1 Antibody (Center) AP22210c
    All lanes : Anti-TK1 Antibody (Center) at 1:2000 dilution Lane 1: U-2OS whole cell lysate Lane 2: SW620 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession P04183
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 25469 Da
Additional Information
Gene ID 7083
Other Names Thymidine kinase, cytosolic, 2.7.1.21, TK1
Target/Specificity This TK1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 139-173 amino acids from the Central region of human TK1.
Dilution FC~~1:25
IHC-P~~1:25
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTK1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TK1
Cellular Location Cytoplasm.
Research Areas
Citations (0)

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References

Bradshaw H.D. Jr.,et al.Mol. Cell. Biol. 4:2316-2320(1984).
Flemington E.,et al.Gene 52:267-277(1987).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Kreidberg J.A.,et al.Mol. Cell. Biol. 6:2903-2909(1986).

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$ 295.00
$ 99.00
Cat# AP22210c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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