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POLR2F Antibody (N-Term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P61218 |
| Other Accession | Q32PE0, P61217, P61219, Q5R592, O88828 |
| Reactivity | Mouse |
| Predicted | Bovine, Hamster, Rat |
| Host | Rabbit |
| Clonality | polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 14478 Da |
| Gene ID | 5435 |
|---|---|
| Other Names | DNA-directed RNA polymerases I, II, and III subunit RPABC2, RNA polymerases I, II, and III subunit ABC2, DNA-directed RNA polymerase II subunit F, DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide, RPABC14.4, RPB14.4, RPB6 homolog, RPC15, POLR2F, POLRF |
| Target/Specificity | This POLR2F antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 27-61 amino acids from human POLR2F. |
| Dilution | WB~~1:2000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | POLR2F Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | POLR2F (HGNC:9193) |
|---|---|
| Synonyms | POLRF |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non- coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPABC2 is part of the clamp element and together with parts of POLR2A/RPB1 and POLR2B/RPB2 forms a pocket to which the POLR2D/RPB4-POLR2G/RPB7 subcomplex binds. |
| Cellular Location | Nucleus. Nucleus, nucleolus |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and together with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds (By similarity).
References
Acker J.,et al.DNA Seq. 4:329-331(1994).
Pusch C.,et al.Genomics 34:440-442(1996).
Collins J.E.,et al.Genome Biol. 5:R84.1-R84.11(2004).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Dunham I.,et al.Nature 402:489-495(1999).
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