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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   EEF2K (Ser366) Antibody   

EEF2K (Ser366) Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - EEF2K (Ser366) Antibody AP22222a
    All lanes : Anti-EEF2k Antibody at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: C6 whole cell lysate Lane 4: Hela whole cell lysate Lane 5: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O00418
Reactivity Human, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 82144 Da
Additional Information
Gene ID 101930123;29904
Other Names Eukaryotic elongation factor 2 kinase, eEF-2 kinase, eEF-2K, 2.7.11.20, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, EEF2K
Target/Specificity This EEF2k (Ser366) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 337-371 amino acids from human EEF2k.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEEF2K (Ser366) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EEF2K
Function Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
Research Areas
Citations (0)

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Background

Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.

References

Ryazanov A.G.,et al.Proc. Natl. Acad. Sci. U.S.A. 94:4884-4889(1997).
Martin J.,et al.Nature 432:988-994(2004).
Pavur K.S.,et al.Biochemistry 39:12216-12224(2000).
Knebel A.,et al.EMBO J. 20:4360-4369(2001).
Wang X.,et al.EMBO J. 20:4370-4379(2001).

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$ 295.00
$ 99.00
Cat# AP22222a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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