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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   MYPT1 (Thr696) Antibody   

MYPT1 (Thr696) Antibody

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - MYPT1 (Thr696) Antibody AP22225a
    All lanes : Anti-MYPT Antibody at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: C2C12 whole cell lysate Lane 3: C6 whole cell lysate Lane 4: Hela whole cell lysate Lane 5: HT-29 whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 115 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O14974
Other Accession Q90623, Q6DRG7, Q9DBR7, Q10728
Reactivity Human, Mouse, Rat
Predicted Chicken, Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 115281 Da
Additional Information
Gene ID 4659
Other Names Protein phosphatase 1 regulatory subunit 12A, Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1, Protein phosphatase myosin-binding subunit, PPP1R12A, MBS, MYPT1
Target/Specificity This MYPT1 (Thr696) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 669-702 amino acids from the human MYPT1.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMYPT1 (Thr696) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPP1R12A
Synonyms MBS, MYPT1
Function Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN- dependent suppression of HIF1A activity.
Cellular Location Cytoplasm. Note=Along actomyosin filaments and stress fibers
Tissue Location Expressed in striated muscles, specifically in type 2a fibers (at protein level).
Research Areas
Citations (0)

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Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN- dependent suppression of HIF1A activity.


Takahashi N.,et al.Genomics 44:150-152(1997).
Guo J.H.,et al.Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases.
Xia D.,et al.Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Scherer S.E.,et al.Nature 440:346-351(2006).

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$ 295.00
$ 99.00
Cat# AP22225a
(40 western blots)
Availability: In Stock
Bulk Size
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