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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   CH25H Antibody (N-Term)   

CH25H Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CH25H Antibody (N-Term) AP22272a
    All lanes : Anti-CH25H Antibody (N-Term) at 1:1000-1:2000 dilution Lane 1: Human brain lysate Lane 2: Mouse heart lysate Lane 3: Mouse lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 32 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O95992
Other Accession Q4G1G8
Reactivity Human, Mouse
Predicted Pig
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 31745 Da
Additional Information
Gene ID 9023
Other Names Cholesterol 25-hydroxylase, 1.14.99.38, Cholesterol 25-monooxygenase, h25OH, CH25H
Target/Specificity This CH25H antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 47-76 amino acids from human CH25H.
Dilution WB~~1:1000-1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCH25H Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CH25H
Function Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed:9852097). Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.
Cellular Location Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9Z0F5}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q9Z0F5}
Research Areas
Citations (0)

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Background

Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25- hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.

References

Lund E.G.,et al.J. Biol. Chem. 273:34316-34327(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Deloukas P.,et al.Nature 429:375-381(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Riemenschneider M.,et al.Neurobiol. Aging 25:1305-1308(2004).

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$ 295.00
$ 99.00
Cat# AP22272a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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