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beta Tubulin Antibody
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC, E |
|---|---|
| Primary Accession | P99024 |
| Other Accession | Q17299, P12456, P09203, Q24560, Q9YHC3, Q27U48, Q25009, O17449, P36221, Q13885, Q4R5B3, Q7TMM9, P85108, Q6B856, Q9BVA1, Q9CWF2, Q3KRE8, P32882, P83130, P61858, P61857, Q9NFZ6, P13602, Q2T9S0, P09206, P08841, Q13509, Q60HC2, Q9ERD7, Q4QRB4, Q3ZBU7, P04350 |
| Reactivity | Mouse |
| Predicted | C.Elegans, Chicken, Drosophila, Human, Monkey, Rat, Bovine, Xenopus, Hamster, Pig |
| Host | Rabbit |
| Clonality | polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 49671 Da |
| Gene ID | 22154 |
|---|---|
| Other Names | Tubulin beta-5 chain, Tubb5 |
| Target/Specificity | This beta Tubulin antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 298-328 amino acids from the mouse region of mouse beta Tubulin. |
| Dilution | WB~~1:2000 FC~~1:25 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | beta Tubulin Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Tubb5 |
|---|---|
| Function | Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. |
| Cellular Location | Cytoplasm, cytoskeleton |
| Tissue Location | Ubiquitously expressed with highest levels in spleen, thymus and immature brain. Expressed in embryonic brain, including throughout the developing cortex and in the subventricular zone. Also found in radial glial cells, intermediate progenitors, migrating neurons and postmitotic neurons (PubMed:23246003). Expressed in skin and developing hair follicle (PubMed:26637975) |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
References
Wang D.,et al.J. Cell Biol. 103:1903-1910(1986).
Carninci P.,et al.Science 309:1559-1563(2005).
Church D.M.,et al.PLoS Biol. 7:E1000112-E1000112(2009).
Lubec G.,et al.Submitted (JUL-2007) to UniProtKB.
Lewis S.A.,et al.J. Cell Biol. 101:852-861(1985).
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