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SLC17A6 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • FC - SLC17A6 Antibody (Center) AP22344c
    Overlay histogram showing U-87 MG cells stained with AP22344c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22344c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - SLC17A6 Antibody (Center) AP22344c
    Anti-SLC17A6 Antibody (Center) at 1:2000 dilution + Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 64 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession Q9P2U8
Other Accession A6QLI1, Q8BLE7, Q9JI12
Reactivity Human, Rat
Predicted Bovine, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 64392 Da
Additional Information
Gene ID 57084
Other Names Vesicular glutamate transporter 2, VGluT2, Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 6, SLC17A6, DNPI, VGLUT2
Target/Specificity This SLC17A6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 255-289 amino acids of human SLC17A6.
Dilution FC~~1:25
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSLC17A6 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SLC17A6
Synonyms DNPI, VGLUT2
Function Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
Cellular Location Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000250|UniProtKB:Q8BLE7}. Membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome {ECO:0000250|UniProtKB:Q8BLE7}
Tissue Location Predominantly expressed in adult brain. Expressed in amygdala, caudate nucleus, cerebral cortex, frontal lobe, hippocampus, medulla, occipital lobe, putamen, spinal cord, substantia nigra, subthalamic nucleus, temporal lobe and thalamus
Research Areas
Citations (0)

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Background

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.

References

Aihara Y.,et al.J. Neurochem. 74:2622-2625(2000).
Taylor T.D.,et al.Nature 440:497-500(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Takamori S.,et al.J. Neurosci. 21:RC182-RC182(2001).
Sjoeblom T.,et al.Science 314:268-274(2006).

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$ 295.00
$ 99.00
Cat# AP22344c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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