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LGG-1/Atg8 antibodies

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - LGG-1/Atg8 antibodies AP22381b
    For each well, 30 worms were picked into Laemmli buffer and boiled at 95℃ for 15 min. Lysates were then used for SDS PAGE/western blotting. All antibodies (including 3 new ones to be tested for Abgent) were diluted 1:1000 in 1% milk in TBST. #83 was used as control (original Abgent #7952).DA2123 is a transgenic C. elegans strain that expresses GFP-tagged LGG-1 protein (Kang et al., Genes & Develop, 2007);#97 RB59577, #98 RB59578, and #99 RB59579 can recognize LGG-1 and GFP::LGG-1, albeit not as potently as the original Abgent antibody.(Data courtesy of Dr. Yongzhi Yang, Hansen lab SBP Institute, July 2018.)
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q09490
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 14764 Da
Antigen Region 20-55 aa
Additional Information
Gene ID 174050
Other Names Protein lgg-1, lgg-1
Target/Specificity This lgg-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-55 amino acids from the center region of CAEEL lgg-1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLGG-1/Atg8 antibodies is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name lgg-1 {ECO:0000312|WormBase:C32D5.9}
Function Ubiquitin-like modifier involved in autophagy and essential for dauer development and life-span extension (PubMed:12958363, PubMed:20523114). Involved in allophagy, which is an autophagic process in which paternal mitochondria and organelles are degraded during fertilization, and moreover is required for the formation of lgg-2-positive allophagic autophagosomes in embryos (PubMed:24374177). Plays a role in the distribution and clearance of germ cell specific P-granules from somatic cells (PubMed:19167332). Required for normal survival when exposed to pathogenic bacteria S.typhimurium probably by promoting autophagic degradation of intracellular S.typhimurium (PubMed:19667176). Plays a role in mitophagy (PubMed:25896323).
Cellular Location Preautophagosomal structure Cytoplasmic vesicle, autophagosome. Cytoplasmic vesicle, autophagosome membrane. Lysosome lumen. Mitochondrion. Cytoplasm. Note=In embryos, diffuse cytoplasmic localization with some areas displaying a more punctate distribution (PubMed:19377305, PubMed:19167332, PubMed:24374177, PubMed:28806108). Specifically, upon fertilization localizes to autophagosomes around the male pronucleus (PubMed:24374177) During the first embryonic divisions and after the 25-cell stage, localizes to a large population of autophagosomes, with another smaller population of autophagosomes containing both lgg-1 and lgg-2 (PubMed:24374177). Localization to autophagosomes is dependent on atg-7 (PubMed:24374177). Co-localizes with sepa-1 in cytoplasmic aggregates (PubMed:19167332, PubMed:28806108)
Tissue Location Expressed in PLML touch receptor neuron and in the ventral nerve cord.
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Discontinued
Cat# AP22381b
Size:
(40 western blots)

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