|Application ||WB, IF, E|
|Calculated MW||174764 Da|
|Other Names||Glycogen debranching enzyme, Glycogen debrancher, 4-alpha-glucanotransferase, Oligo-1, 4-1, 4-glucantransferase, Amylo-alpha-1, 6-glucosidase, Amylo-1, 6-glucosidase, Dextrin 6-alpha-D-glucosidase, AGL, GDE|
|Target/Specificity||This AGL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human AGL.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||AGL Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4- alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6- glucosidase in glycogen degradation.|
|Cellular Location||Cytoplasm. Note=Under glycogenolytic conditions localizes to the nucleus|
|Tissue Location||Liver, kidney and lymphoblastoid cells express predominantly isoform 1; whereas muscle and heart express not only isoform 1, but also muscle-specific isoform mRNAs (isoforms 2, 3 and 4). Isoforms 5 and 6 are present in both liver and muscle|
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Provided below are standard protocols that you may find useful for product applications.
AGL is a glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in the AGL gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing.
Horinishi, A., et al., J. Hum. Genet. 47(2):55-59 (2002).
Shen, J., et al., Hum. Mutat. 9(1):37-40 (1997).
Bao, Y., et al., Genomics 38(2):155-165 (1996).
Shen, J., et al., J. Clin. Invest. 98(2):352-357 (1996).
Yang, B.Z., et al., J. Biol. Chem. 267(13):9294-9299 (1992).
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