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>   home   >   Products   >   Primary Antibodies   >   Ribophorin (RPN1) Antibody (C-term)   

Ribophorin (RPN1) Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Ribophorin (RPN1) Antibody (C-term) AP2409B
    All lanes : Anti-RPN1 Antibody (D546) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 69 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Ribophorin (RPN1) Antibody (C-term) AP2409B
    The anti-RPN1 Pab (Cat. #AP2409b) is used in Western blot to detect RPN1 in HeLa cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04843
Other Accession P07153, Q91YQ5, Q4R4T0
Reactivity Human, Mouse
Predicted Monkey, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 68569 Da
Antigen Region 531-561 aa
Additional Information
Gene ID 6184
Other Names Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, Ribophorin I, RPN-I, Ribophorin-1, RPN1
Target/Specificity This Ribophorin (RPN1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 531-561 amino acids from the C-terminal region of human Ribophorin (RPN1).
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRibophorin (RPN1) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPN1
Function Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
Cellular Location Endoplasmic reticulum membrane; Single-pass type I membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Tissue Location Expressed in all tissues tested.
Citations (0)

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Ribophorins 1 and 2 are abundant and highly conserved glycoproteins residing in the endoplasic reticulum, that participate in ribosome binding. Mammalian oligosaccharyltransferase activity is associated with a protein complex composed of RPN1, RPN2, and an oligosaccharyltransferase protein. RPN1 is a component of the proteasome base. The ubiquitin-like (UBL) domain of recombinant Rad23 interacts with proteasomes through the leucine-rich repeat domain of RPN1. The RPN1 gene maps to chromosome 3 in somatic cell hybrids, and the RPN2 gene maps to chromosome 20 by in situ hybridization.


Fu, J., et al., J. Biol. Chem. 275(6):3984-3990 (2000). Pekarsky, Y., et al., Cancer Res. 57(18):3914-3919 (1997). Crimaudo, C., et al., EMBO J. 6(1):75-82 (1987).

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$ 295.00
$ 99.00
Cat# AP2409B
(40 western blots)
Availability: In Stock
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