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MGAT3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - MGAT3 Antibody (N-term) AP2411A
    Western blot analysis of MGAT3 antibody (N-term) (Cat.# AP2411a) in mouse spleen tissue lysates (35ug/lane). MGAT3 (arrow) was detected using the purified Pab.
  • IHC-P - MGAT3 Antibody (N-term) AP2411A
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q09327
Other Accession NP_002400
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 61313 Da
Antigen Region 72-102 aa
Additional Information
Gene ID 4248
Other Names Beta-1, 4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III, GNT-III, GlcNAc-T III, N-acetylglucosaminyltransferase III, MGAT3, GGNT3
Target/Specificity This MGAT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-102 amino acids from the N-terminal region of human MGAT3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMGAT3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MGAT3
Synonyms GGNT3
Function It is involved in the regulation of the biosynthesis and biological function of glycoprotein oligosaccharides. Catalyzes the addition of N-acetylglucosamine in beta 1-4 linkage to the beta-linked mannose of the trimannosyl core of N-linked sugar chains. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
Cellular Location Golgi apparatus membrane; Single-pass type II membrane protein
Citations (0)

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There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. MGAT3 (N-acetylglucosaminyltransferase III) transfers a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides and produces a bisecting GlcNAc. Expression of this gene may be controlled by a multiple-promoter system.


Shibukawa, Y., et al., J. Biol. Chem. 278(5):3197-3203 (2003).
Koyama, N., et al., Eur. J. Biochem. 238(3):853-861 (1996).
Kim, Y.J., et al., Gene 170(2):281-283 (1996).
Ihara, Y., et al., J. Biochem. 113(6):692-698 (1993).

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Cat# AP2411A
(40 western blots)
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