FNTB Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
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Primary Accession | P49356 |
Other Accession | Q02293, P49355, NP_002019 |
Reactivity | Human |
Predicted | Bovine, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 48774 Da |
Antigen Region | 6-36 aa |
Gene ID | 100529261;2342 |
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Other Names | Protein farnesyltransferase subunit beta, FTase-beta, CAAX farnesyltransferase subunit beta, Ras proteins prenyltransferase subunit beta, FNTB |
Target/Specificity | This FNTB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-36 amino acids from the N-terminal region of human FNTB. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | FNTB Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | FNTB |
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Function | Essential subunit of the farnesyltransferase complex. Catalyzes the transfer of a farnesyl moiety from farnesyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Eukaryotic cells contain 3 different types of prenyltransferases that attach either a farnesyl group (15 carbons) or a geranylgeranyl group (20 carbons) in thioether linkage to C-terminal cysteine residues in a variety of proteins. These posttranslational modifications provide a mechanism for membrane localization of proteins that lack a transmembrane domain. CAAX farnesyltransferase (FTase) attaches a farnesyl group from farnesyl pyrophosphate to cysteine residues at the fourth position from the C terminus of proteins that end in the CAAX box, where C is cysteine, A is usually but not always an aliphatic amino acid, and X is typically methionine or serine. This enzyme has the ability to farnesylate peptides as short as 4 residues in length that conform to the CAAX consensus sequence. The gene for the beta subunit of CAAX farnesyltransferase (FNTB) has been pinpointed to 14q23-q24 by Southern blot hybridization and PCR analyses of panels of human/Chinese hamster somatic cell hybrid lines and by fluorescence chromosomal in situ hybridization.
References
Lobell, R.B., et al., Cancer Res. 61(24):8758-8768 (2001).
Wang, T., et al., Science 271(5252):1120-1122 (1996).
Andres, D.A., et al., Genomics 18(1):105-112 (1993).
Omer, C.A., et al., Biochemistry 32(19):5167-5176 (1993).
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