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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   FNTB Antibody (N-term)   

FNTB Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - FNTB Antibody (N-term) AP2414a
    Western blot analysis of FNTB (arrow) using FNTB Antibody (N-term) (Cat.#AP2414a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FNTB gene (Lane 2) (Origene Technologies).
  • IHC-P - FNTB Antibody (N-term) AP2414a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P49356
Other Accession Q02293, P49355, NP_002019
Reactivity Human
Predicted Bovine, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 48774 Da
Antigen Region 6-36 aa
Additional Information
Gene ID 100529261;2342
Other Names Protein farnesyltransferase subunit beta, FTase-beta, CAAX farnesyltransferase subunit beta, Ras proteins prenyltransferase subunit beta, FNTB
Target/Specificity This FNTB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-36 amino acids from the N-terminal region of human FNTB.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFNTB Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FNTB
Function Essential subunit of the farnesyltransferase complex. Catalyzes the transfer of a farnesyl moiety from farnesyl diphosphate to a cysteine at the fourth position from the C- terminus of several proteins having the C-terminal sequence Cys- aliphatic-aliphatic-X.
Research Areas
Citations (0)

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Background

Eukaryotic cells contain 3 different types of prenyltransferases that attach either a farnesyl group (15 carbons) or a geranylgeranyl group (20 carbons) in thioether linkage to C-terminal cysteine residues in a variety of proteins. These posttranslational modifications provide a mechanism for membrane localization of proteins that lack a transmembrane domain. CAAX farnesyltransferase (FTase) attaches a farnesyl group from farnesyl pyrophosphate to cysteine residues at the fourth position from the C terminus of proteins that end in the CAAX box, where C is cysteine, A is usually but not always an aliphatic amino acid, and X is typically methionine or serine. This enzyme has the ability to farnesylate peptides as short as 4 residues in length that conform to the CAAX consensus sequence. The gene for the beta subunit of CAAX farnesyltransferase (FNTB) has been pinpointed to 14q23-q24 by Southern blot hybridization and PCR analyses of panels of human/Chinese hamster somatic cell hybrid lines and by fluorescence chromosomal in situ hybridization.

References

Lobell, R.B., et al., Cancer Res. 61(24):8758-8768 (2001).
Wang, T., et al., Science 271(5252):1120-1122 (1996).
Andres, D.A., et al., Genomics 18(1):105-112 (1993).
Omer, C.A., et al., Biochemistry 32(19):5167-5176 (1993).

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$ 295.00
$ 99.00
Cat# AP2414a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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