- CITATIONS: 1
|Application ||WB, IHC-P, E|
|Other Accession||Q04631, Q61239, P29702|
|Predicted||Bovine, Mouse, Rat|
|Calculated MW||44409 Da|
|Antigen Region||330-360 aa|
|Other Names||Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha, CAAX farnesyltransferase subunit alpha, FTase-alpha, Ras proteins prenyltransferase subunit alpha, Type I protein geranyl-geranyltransferase subunit alpha, GGTase-I-alpha, FNTA|
|Target/Specificity||This FNTA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 330-360 amino acids from the C-terminal region of human FNTA.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||FNTA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential subunit of both the farnesyltransferase and the geranylgeranyltransferase complex. Contributes to the transfer of a farnesyl or geranylgeranyl moiety from farnesyl or geranylgeranyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. May positively regulate neuromuscular junction development downstream of MUSK via its function in RAC1 prenylation and activation.|
Provided below are standard protocols that you may find useful for product applications.
FNTA, also known as CAAX farnesyltransferase (FTase), attaches a farnesyl group from farnesyl pyrophosphate to cysteine residues at the fourth position from the C terminus of proteins that end in the so-called CAAX box, where C is cysteine, A is usually but not always an aliphatic amino acid, and X is typically methionine or serine. This type of posttranslational modification provides a mechanism for membrane localization of proteins that lack a transmembrane domain. This enzyme has the remarkable property of farnesylating peptides as short as four residues in length that conform to the CAAX consensus sequence. FNTA is also a specific cytoplasmic interactor of the transforming growth factor-beta and activin type I receptors. It is likely to be a key component of the signaling pathway which involves p21ras, an important substrate for farnesyltransferase.
Wang, T., et al., Science 271(5252):1120-1122 (1996).
Zhang, F.L., et al., J. Biol. Chem. 269(5):3175-3180 (1994).
Andres, D.A., et al., Genomics 18(1):105-112 (1993).
Omer, C.A., et al., Biochemistry 32(19):5167-5176 (1993).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.