|Application ||WB, IHC-P, E|
|Other Accession||Q9CXY9, Q3MHZ7|
|Calculated MW||45252 Da|
|Antigen Region||266-297 aa|
|Other Names||GPI-anchor transamidase, GPI transamidase, 3---, GPI8 homolog, hGPI8, Phosphatidylinositol-glycan biosynthesis class K protein, PIG-K, PIGK, GPI8|
|Target/Specificity||This GPI8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-297 amino acids from the C-terminal region of human GPI8.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GPI8 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with the substrate protein.|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass type I membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
GPI8 is a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins.
Ohishi, K., et al., J. Biol. Chem. 278(16):13959-13967 (2003).
Vainauskas, S., et al., J. Biol. Chem. 277(34):30535-30542 (2002).
Ohishi, K., et al., EMBO J. 20(15):4088-4098 (2001).
Meyer, U., et al., Biochemistry 39(12):3461-3471 (2000).
Yu, J., et al., Proc. Natl. Acad. Sci. U.S.A. 94(23):12580-12585 (1997).
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