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HSF2 Sumoylation Site AntibodyPurified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP2502a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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HSF2 Sumoylation Site Antibody - Product info | |
| Application | WB, IHC
|
| Primary Accession | Q03933 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 60348 Da |
HSF2 Sumoylation Site Antibody - Additional info | |
| Gene ID 3298 | |
| Other Names HSF2; HSTF2; Heat shock factor protein 2; Heat shock transcription factor 2 | |
| Target/Specificity This HSF2 Sumoylation Site antibody is generated from rabbits immunized with the KLH conjugated synthetic peptide VHIDSGIVKQERDGP containing a predicted sumoylation site from the N-terminal region of human HSF2. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions HSF2 Sumoylation Site Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
HSF2 Sumoylation Site Antibody - Protein Information | |
| Name HSF2 | |
| Synonyms HSTF2 | |
| Function DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked | |
| Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth and moves to the nucleus upon activation | |
HSF2 Sumoylation Site Antibody - Related products
AP2502a: HSF2 Sumoylation Site Antibody
DC14202: Human HSF2 cDNA Clone
LY12174a: HSF2 Over-expression Lysate
HSF2 Sumoylation Site Antibody - Application data
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Western blot analysis of anti-HSF2 Sumoylation Site Antibody (Cat.#AP2502a) in HepG2 cell line lysates (35ug/lane). HSF2 (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human testis tissue reacted with HSF2 Sumoylation Site antibody (Cat.#AP2502a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
HSF2 Sumoylation Site Antibody - Research Areas
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BACKGROUND
HSF2 is a DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. The protein is found as a DNA-binding homotrimer in stressed or heat shocked cells, and otherwise found as a homodimer. HSF2 is cytoplasmic during normal growth and moves to the nucleus upon activation. Sumoylation of HSF2 hinders HSF2 DNA-binding activity, without affecting its oligomerization, and is an example of negative regulation of gene expression via sumoylation.
REFERENCES
He, H., et al., J. Biol. Chem. 278(37):35465-35475 (2003). Nykanen, P., et al., Cell Stress Chaperones 6(4):377-385 (2001). Sarge, K.D., et al., Genes Dev. 5(10):1902-1911 (1991). Schuetz, T.J., et al., Proc. Natl. Acad. Sci. U.S.A. 88(16):6911-6915 (1991).
