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RanGAP1 (Ran-GTPase) Antibody (Sumoylation Site Specific)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP2503a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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RanGAP1 (Ran-GTPase) Antibody (Sumoylation Site Specific) - Product info | |
| Application | WB
|
| Primary Accession | P46060 |
| Other Accession | NP_002874 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 63542 Da |
RanGAP1 (Ran-GTPase) Antibody (Sumoylation Site Specific) - Additional info | |
| Gene ID 5905 | |
| Other Names RANGAP1; KIAA1835; SD; Ran GTPase-activating protein 1 | |
| Target/Specificity This RanGAP1 (Ran-GTPase) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human Ran-GTPase around the sumoylation site. | |
| Dilution WB~~1:250~500WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions RanGAP1 (Ran-GTPase) Antibody (Sumoylation Site Specific) is for research use only and not for use in diagnostic or therapeutic procedures. | |
RanGAP1 (Ran-GTPase) Antibody (Sumoylation Site Specific) - Protein Information | |
| Name RANGAP1 | |
| Synonyms KIAA1835, SD | |
| Function GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state | |
| Cellular Location Cytoplasm. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Chromosome, centromere, kinetochore (By similarity). Cytoplasm, cytoskeleton, spindle pole (By similarity). Note=Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase Mitotic location also requires sumoylation | |
| Tissue Location Highly expressed in brain, thymus and testis. | |
RanGAP1 (Ran-GTPase) Antibody (Sumoylation Site Specific) - Related products
AP13776a: RANGAP1 Antibody (N-term)
AP2503a: RanGAP1 (Ran-GTPase) Antibody (Sumoylation Site Specific)
DC03740: Human RANGAP1 cDNA Clone
BP2503a: Ran-GTPase Sumoylation site Antibody Blocking Peptide
RanGAP1 (Ran-GTPase) Antibody (Sumoylation Site Specific) - Application data
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Western blot analysis of anti-Ran-GTPase Sumoylation site Pab (AP2503a) in HepG2 cell line lysate. Ran-GTPase Sumoylation site(arrow) was detected using the purified Pab.
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Western blot analysis of Ran-GTPase (arrow) using rabbit polyclonal Ran-GTPase Antibody (Sumo) (Cat. #AP2503a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Ran-GTPase gene.
RanGAP1 (Ran-GTPase) Antibody (Sumoylation Site Specific) - Research Areas
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BACKGROUND
RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle.
REFERENCES
Bischoff, F.R., et al., Proc. Natl. Acad. Sci. U.S.A. 92(5):1749-1753 (1995). Bischoff, F.R., et al., Proc. Natl. Acad. Sci. U.S.A. 91(7):2587-2591 (1994). Matunis, M.J., et al., J. Cell Biol. 135 (6 Pt 1), 1457-1470 (1996).
