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PML Antibody (Sumoylation Site Specific)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Alternative | |||
| AP2504a | AP2504b | DISCONTINED INQUIRE CLICK INQUIRE Add to cart | ||
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
PML Antibody (Sumoylation Site Specific) - Product info | |
| Application | WB, IF, FC
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| Primary Accession | P29590 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 97551 Da |
PML Antibody (Sumoylation Site Specific) - Additional info | |
| Gene ID 5371 | |
| Other Names PML; MYL; RNF71; TRIM19; Protein PML; Promyelocytic leukemia protein; RING finger protein 71; Tripartite motif-containing protein 19 | |
| Target/Specificity This PML antibody is generated from rabbits immunized with the KLH conjugated synthetic peptide HQWFLKHEARPLAELR containing a predicted sumoylation site from the N-terminal region of human PML. | |
| Dilution WB~~1:100~500WB~~1:1000 IF~~1:10~50 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions PML Antibody (Sumoylation Site Specific) is for research use only and not for use in diagnostic or therapeutic procedures. | |
PML Antibody (Sumoylation Site Specific) - Protein Information | |
| Name PML | |
| Synonyms MYL, RNF71, TRIM19 | |
| Function Key component of PML nuclear bodies that regulate a large number of cellular processes by facilitating post- translational modification of target proteins, promoting protein- protein contacts, or by sequestering proteins. Functions as tumor suppressor. Required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. Plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. Plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. Required for normal immunity to microbial infections. Plays a role in antiviral response. In the cytoplasm, plays a role in TGFB1-dependent processes. Regulates p53/TP53 levels by inhibiting its ubiquitination and proteasomal degradation. Regulates activation of p53/TP53 via phosphorylation at 'Ser-20'. Sequesters MDM2 in the nucleolus after DNA damage, and thereby inhibits ubiquitination and degradation of p53/TP53 Regulates translation of HIF1A by sequestering MTOR, and thereby plays a role in neoangiogenesis and tumor vascularization Regulates RB1 phosphorylation and activity. Required for normal development of the brain cortex during embryogenesis. Can sequester herpes virus and varicella virus proteins inside PML bodies, and thereby inhibit the formation of infectious viral particles. Regulates phosphorylation of ITPR3 and plays a role in the regulation of calcium homeostasis at the endoplasmic reticulum (By similarity). Regulates transcription activity of ELF4 Inhibits specifically the activity of the tetrameric form of PKM Together with SATB1, involved in local chromatin-loop remodeling and gene expression regulation at the MHC-I locus. Regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitination | |
| Cellular Location Nucleus, nucleoplasm. Cytoplasm. Nucleus, PML body. Nucleus, nucleolus. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Note=Sumoylated forms localize to the PML nuclear bodies The B1 box and the RING finger are also required for this nuclear localization. Isoforms lacking a nuclear localization signal are cytoplasmic. Detected in the nucleolus after DNA damage Sequestered in the cytoplasm by interaction with rabies virus phosphoprotein | |
PML Antibody (Sumoylation Site Specific) - Related products
AP2504a: PML Antibody (Sumoylation Site Specific)
AP2504b: PML Sumoylation Site Antibody
BP2504a: PML Sumoylation Site Antibody Blocking Peptide
PML Antibody (Sumoylation Site Specific) - Application data
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Western blot analysis of PML Sumoylation Site Pab (Cat.# AP2504a) in K562 cell line lysates. Nonsumoylated PML (arrow) was detected using the purified Pab.
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Confocal immunofluorescent analysis of PML Antibody (Sumoylation Site Specific) (Cat#AP2504a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
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PML Antibody (Sumoylation Site Specific) (Cat. #AP2504a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
PML Antibody (Sumoylation Site Specific) - Research Areas
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BACKGROUND
PML is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL).
REFERENCES
Puccetti, E., et al., Oncogene 22(44):6900-6908 (2003). Favre, M., et al., J. Acquir. Immune Defic. Syndr. 34(2):127-133 (2003). Kawai, T., et al., Mol. Cell. Biol. 23(17):6174-6186 (2003). Louria-Hayon, I., et al., J. Biol. Chem. 278(35):33134-33141 (2003). Moller, A., et al., Cancer Res. 63(15):4310-4314 (2003).
