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IKB alpha (IKBA) AntibodyPurified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP2506a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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- Backgrounds
IKB alpha (IKBA) Antibody - Product info | |
| Application | WB
|
| Primary Accession | P25963 |
| Other Accession | NP_065390 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 35609 Da |
IKB alpha (IKBA) Antibody - Additional info | |
| Gene ID 4792 | |
| Other Names NFKBIA; IKBA; MAD3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; Major histocompatibility complex enhancer-binding protein MAD3 | |
| Target/Specificity This IKBA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human IKB-alpha around the sumoylation site. | |
| Dilution WB~~1:100~500WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions IKB alpha (IKBA) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
IKB alpha (IKBA) Antibody - Protein Information | |
| Name NFKBIA | |
| Synonyms IKBA, MAD3, NFKBI | |
| Function Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription | |
| Cellular Location Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export (By similarity) | |
IKB alpha (IKBA) Antibody - Related products
AP2506a: IKB alpha (IKBA) Antibody
AP3666a: Phospho-NFKBIA-S32/36
AP3714a: Phospho-NFKBIA (Ser32) Antibody
AP7981a: NFKBIA Antibody (Y181)
AP7981b: NFKBIA Antibody ( S32/36 )
AP7981d: NFKBIA (Ser32) Antibody
RI13579: NFKBIA predesign siRNA
DC01578: Human NFKBIA cDNA Clone
LY11790a: NFKBIA Over-expression Lysate
BP2506a: IKBa Sumoylation site Antibody Blocking Peptide
BP3666a: Phospho-NFKBIA-S32/36 Blocking Peptide
BP3714a: Phospho-NFKBIA (Ser32) Antibody Blocking Peptide
BP7981a: NFKBIA Antibody (Y181) Blocking Peptide
BP7981b: NFKBIA Antibody ( S32/36 ) Blocking Peptide
BP7981d: Phospho-NFKBIA (Ser32) Antibody Blocking Peptide
AJ1389a: IkappaB alpha Antibody
AJ1389b: IkappaB-alpha Antibody
AJ1389c: IkappaB-alpha Antibody Phospho (pY42)
IKB alpha (IKBA) Antibody - Application data
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The anti-IKB-alpha N-term Pab (Cat. #AP2506a) is used in Western blot to detect IKB-alpha in Jurkat cell lysate.
IKB alpha (IKBA) Antibody - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).
REFERENCES
Miskolci, V., et al., Arch. Biochem. Biophys. 417(1):44-52 (2003). Kim, Y.S., et al., J. Biol. Chem. 278(31):28462-28469 (2003). Parcellier, A., et al., Mol. Cell. Biol. 23(16):5790-5802 (2003). Takada, Y., et al., J. Biol. Chem. 278(26):24233-24241 (2003). Place, R.F., et al., J. Cell. Physiol. 195(3):470-478 (2003).
