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AP-1 Sumoylation Site AntibodyPurified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP2507b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
AP-1 Sumoylation Site Antibody - Product info | |
| Application | WB, IHC, FC
|
| Primary Accession | P05412 |
| Other Accession | P17325, P56432, P05627, P18870, O77627 |
| Reactivity | Human |
| Predicted | Bovine, Chicken, Mouse, Pig, Rat |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 35676 Da |
AP-1 Sumoylation Site Antibody - Additional info | |
| Gene ID 3725 | |
| Other Names JUN; Transcription factor AP-1; Activator protein 1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39 | |
| Target/Specificity This AP-1 Sumoylation Site antibody is generated from rabbits immunized with the KLH conjugated synthetic peptide HPRLQALKEEPQTVPE containing a predicted sumoylation site from the C-terminal region of human AP-1. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions AP-1 Sumoylation Site Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
AP-1 Sumoylation Site Antibody - Protein Information | |
| Name JUN | |
| Function Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation | |
| Cellular Location Nucleus. | |
AP-1 Sumoylation Site Antibody - Related products
AP1984b: JUN Antibody (C-term R272)
AP1984i: JUN Antibody (C-term)
AP1984k: JUN Antibody (C-term E248)
AP2507b: AP-1 Sumoylation Site Antibody
AP3072a: Phospho-cJun-S63 Antibody
AP3073a: Phospho-cJun-S63 Antibody
AP3075a: Phospho-cJun-S73 Antibody
AP3292a: Phospho-JUN-T239 Antibody
AP3293a: Phospho-JUN-Y170 Antibody
AP3294a: Phospho-JUN-T243 Antibody
AP3295a: Phospho-JUN-T249 Antibody
AP3380a: Phospho-JUN-S63 Antibody
AP3402a: Phospho-JUN-S73 Antibody
BP1984b: JUN Antibody (C-term R272) Blocking Peptide
BP1984c: JUN Antibody (T239) Blocking Peptide
BP1984d: JUN Antibody (S63) Blocking Peptide
BP1984e: JUN Antibody (S73) Blocking Peptide
BP1984f: JUN Antibody(Y170) Blocking Peptide
BP1984g: JUN Antibody (T243) Blocking Peptide
BP1984h: JUN Antibody (T249) Blocking Peptide
BP1984k: JUN Antibody (C-term E248) Blocking peptide
BP2507b: AP-1 Sumoylation site Antibody Blocking Peptide
BP3072a: Phospho-cJun-S63 Antibody Blocking Peptide
BP3073a: Phospho-cJun-S63 Antibody Blocking Peptide
BP3075a: Phospho-cJun-S73 Antibody Blocking Peptide
BP3292a: Phospho-JUN-T239 Antibody Blocking Peptide
BP3293a: Phospho-JUN-Y170 Antibody Blocking Peptide
BP3294a: Phospho-JUN-T243 Antibody Blocking Peptide
BP3295a: Phospho-JUN-T249 Antibody Blocking Peptide
BP3380a: Phospho-JUN-S63 Antibody Blocking Peptide
BP3402a: Phospho-JUN-S73 Antibody Blocking Peptide
AJ1188a: c-Jun Antibody (C-term)
AJ1188b: c-Jun Antibody (N-term)
AJ1188c: c-Jun Antibody Phospho (pS63)
AP-1 Sumoylation Site Antibody - Application data
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The AP-1 polyclonal antibody (Cat. #AP2507b) is used in Western blot to detect nonsumoylated AP-1 in Jurkat cell lysate.
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Western blot analysis of AP-1(arrow) using rabbit polyclonal AP-1 Antibody (Cat. #AP2507b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AP-1 gene (Lane 2) (Origene Technologies).
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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AP-1 Antibody (Cat. #AP2507b) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
AP-1 Sumoylation Site Antibody - Research Areas
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BACKGROUND
The gene for AP-1 is the putative transforming gene of avian sarcoma virus 17. AP-1 is highly similar to the viral protein, and interacts directly with specific target DNA sequences to regulate gene expression. The AP-1 gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. AP-1 dimer activity is downregulated by SUMO-1, SUMO-2, and SUMO-3.
REFERENCES
Bossis, et al., Mol Cell Biol. 2005 Aug;25(16):6964-79. Perez-Sala, D., et al., J. Biol. Chem. 278(51):51251-51260 (2003). Yang, H., et al., J. Biol. Chem. 278(51):50887-50896 (2003). Toborek, M., et al., J. Neurochem. 84(1):169-179 (2003). Wang, Y.N., et al., J. Biol. Chem. 278(46):45848-45857 (2003).
