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NYREN18 Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP2510a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
NYREN18 Antibody (N-term) - Product info | |
| Application | WB, IHC
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| Primary Accession | Q9Y5A7 |
| Reactivity | Human, Mouse |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 70538 Da |
NYREN18 Antibody (N-term) - Additional info | |
| Gene ID 51667 | |
| Other Names NUB1; NYREN18; NEDD8 ultimate buster 1; Negative regulator of ubiquitin-like proteins 1; Renal carcinoma antigen NY-REN-18 | |
| Target/Specificity This NYREN18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95~124 amino acids from the N-terminal region of human NYREN18. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions NYREN18 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
NYREN18 Antibody (N-term) - Protein Information | |
| Name NUB1 | |
| Synonyms NYREN18 | |
| Function Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2 | |
| Cellular Location Nucleus. Note=Predominantly nuclear. | |
| Tissue Location Widely expressed with lowest expression in the pancreas for isoform 1 and in leukocytes, liver, prostate and skeletal muscle for isoform 2 | |
NYREN18 Antibody (N-term) - Related products
AP2510a: NYREN18 Antibody (N-term)
AP2510b: NYREN18 Antibody (C-term)
NYREN18 Antibody (N-term) - Application data
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The anti-NYREN18 N-term Pab (Cat. #AP2510a) is used in Western blot to detect NYREN18 in mouse liver lysate.
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Western blot analysis of NUB1 (arrow) using NYREN18 Antibody (N-term) (Cat.#AP2510a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NUB1 gene (Lane 2) (Origene Technologies).
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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NYREN18 Antibody (E102) (Cat. #AP2510a) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the NYREN18 antibody detected the NYREN18 protein (arrow).
NYREN18 Antibody (N-term) - Research Areas
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BACKGROUND
NYREN18 is a specific down-regulator of the NEDD8 conjugation system. It recruits NEDD8 and its conjugates to the proteasome for degradation. It also interacts with PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome.
REFERENCES
Tanaka, T., et al., J. Biol. Chem. 278(35):32905-32913 (2003). Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Kito, K., et al., J. Biol. Chem. 276(23):20603-20609 (2001). Kamitani, T., et al., J. Biol. Chem. 276(49):46655-46660 (2001). Scanlan, M.J., et al., Int. J. Cancer 83(4):456-464 (1999).
