EZH1 Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
---|---|
Primary Accession | Q92800 |
Other Accession | A7E2Z2 |
Reactivity | Human |
Predicted | Bovine |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 85271 Da |
Antigen Region | 393-422 aa |
Gene ID | 2145 |
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Other Names | Histone-lysine N-methyltransferase EZH1, ENX-2, Enhancer of zeste homolog 1, EZH1, KIAA0388 |
Target/Specificity | This EZH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393-422 amino acids from the Central region of human EZH1. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | EZH1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | EZH1 |
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Synonyms | KIAA0388 |
Function | Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. |
Cellular Location | Nucleus. Note=Colocalizes with trimethylated 'Lys-27' of histone H3 |
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Background
EZH1 encodes a protein of 747 amino acids that displays 55% amino acid identity overall with the Drosophila homolog.1 The strong sequence conservation suggested potential roles for EZH1 in human development as a transcriptional regulator and as a component of protein complexes that preserve heterochromatin stability. EZH1 is expressed as 2 major transcripts in all adult and fetal human tissues evaluated.. Analysis of an EZH1 cDNA revealed an unusual splicing event involving EZH1 and a tandemly linked gene GPR2 and suggested a potential mechanism for modifying the EZH1 protein in the conserved C-terminal domain. The GPR2 gene maps to 17q21.1-q21.3 in the vicinity of the BRCA1 gene.
References
Ogawa, M., et al., Biochim. Biophys. Acta 1395(2):151-158 (1998).
Abel, K.J., et al., Genomics 37(2):161-171 (1996).
Friedman, L.S., et al., Genomics 25(1):256-263 (1995).
Osborne-Lawrence, S., et al., Genomics 25(1):248-255 (1995).
Brody, L.C., et al., Genomics 25(1):238-247 (1995).
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