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>   home   >   Products   >   Primary Antibodies   >   Cancer   >   EZH2 Antibody (Center)   

EZH2 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - EZH2 Antibody (Center) AP2512c
    Anti-EZH2 Antibody (I418)at 1:1000 dilution + C6 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 85 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - EZH2 Antibody (Center) AP2512c
    The anti-EZH2 Ctr Pab (Cat. #AP2512c) is used in Western blot to detect EZH2 in mouse kidney tissue lysate.
  • IHC-P - EZH2 Antibody (Center) AP2512c
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • IHC-P - EZH2 Antibody (Center) AP2512c
    Formalin-fixed and paraffin-embedded human Testis tissue reacted with EZH2 Antibody (Center)(Cat.#AP2512c), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15910
Other Accession Q61188, Q4R381, Q4V863, Q98SM3
Reactivity Human, Mouse
Predicted Monkey, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 85363 Da
Additional Information
Gene ID 2146
Other Names Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6, EZH2, KMT6
Target/Specificity This EZH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human EZH2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEZH2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EZH2
Synonyms KMT6
Function Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys- 27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.
Cellular Location Nucleus
Tissue Location Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.
Research Areas
Citations ( 0 )


EZH2, SUZ12, and EED form a complex that methylates nucleosomal histone H3 at Lys27. EZH2 contains a SET domain, a signature motif for all known histone lysine methyltransferases except the H3-K79 methyltransferase DOT1, and is therefore likely to be the catalytic subunit. Consequently, EZH2 is thought to regulate gene expression by controlling chromatin structure. Several lines of evidence suggested a critical role for the EZH2 protein during normal and perturbed development of the hematopoietic and central nervous systems. The EZH2 protein has been shown to associate with the VAV1 protooncoprotein and with the XNP protein, the product of a gene associated with mental retardation. Additionally, due to mapping of EZH2 to the 7q35-q36 chromosomal region associated with myeloid disorders, this protein is suggested to participate in the genetic events triggering myeloid leukemia.


Cardoso, C., et al., Hum. Mol. Genet. 7(4):679-684 (1998).
Laible, G., et al., EMBO J. 16(11):3219-3232 (1997).
Hobert, O., et al., Mol. Cell. Biol. 16(6):3066-3073 (1996).
Chen, H., et al., Genomics 38(1):30-37 (1996).

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$ 295.00
Cat# AP2512c
(40 western blots)
Availability: In Stock
Bulk Size
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