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PC2 (CBX4) Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP2514a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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PC2 (CBX4) Antibody (N-term) - Product info | |
| Application | WB, IHC
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| Primary Accession | O00257 |
| Reactivity | Human, Mouse |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 61368 Da |
PC2 (CBX4) Antibody (N-term) - Additional info | |
| Gene ID 8535 | |
| Other Names CBX4; E3 SUMO-protein ligase CBX4; Chromobox protein homolog 4; Polycomb 2 homolog | |
| Target/Specificity This PC2 (CBX4) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88~117 amino acids from the N-terminal region of human CBX4. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions PC2 (CBX4) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
PC2 (CBX4) Antibody (N-term) - Protein Information | |
| Name CBX4 | |
| Function E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression | |
| Cellular Location Nucleus. Nucleus speckle. | |
| Tissue Location Ubiquitous. | |
PC2 (CBX4) Antibody (N-term) - Related products
AP17028a: CBX4 Antibody (N-term)
AP2514a: PC2 (CBX4) Antibody (N-term)
AP2514b: CBX4 Antibody (C-term)
DC03981: Human CBX4 cDNA Clone
LY13976a: CBX4 Over-expression Lysate
PC2 (CBX4) Antibody (N-term) - Application data
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The anti-CBX4 N-term Pab (Cat. #AP2514a) is used in Western blot to detect CBX4 in mouse kidney tissue lysate.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
PC2 (CBX4) Antibody (N-term) - Research Areas
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BACKGROUND
CBX4 is a member of Drosophila Polycomb group gene family. The polycomb group (PcG) genes are essential for maintenance of proper expression patterns of developmental master regulators; changes in expression pf PcG proteins have been associated with cancer. CBX4 is a part of the cellular memory system responsible for the inheritance of gene activity by progeny cells. It participates in maintaining the transcriptionally repressive state of genes. CBX4 is part of acomplex that acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. CBX4 is an E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I.
REFERENCES
Kagey, M.H., et al., Cell 113(1):127-137 (2003). Satijn, D.P., et al., Mol. Cell. Biol. 17(10):6076-6086 (1997).
