|Application ||WB, E|
|Calculated MW||31555 Da|
|Antigen Region||241-271 aa|
|Other Names||Myristoylated alanine-rich C-kinase substrate, MARCKS, Protein kinase C substrate, 80 kDa protein, light chain, 80K-L protein, PKCSL, MARCKS, MACS, PRKCSL|
|Target/Specificity||This MARCKS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-271 amino acids from the C-terminal region of human MARCKS.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MARCKS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.|
|Cellular Location||Cytoplasm, cytoskeleton. Membrane; Lipid-anchor|
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Provided below are standard protocols that you may find useful for product applications.
MARCKS is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.
Rauch, M.E., et al., J. Biol. Chem. 277(16):14068-14076 (2002).
Aderem, A., Biochem. Soc. Trans. 23(3):587-591 (1995).
Rao, P.H., et al., Cytogenet. Cell Genet. 66(4):272-273 (1994).
Taniguchi, H., et al., J. Biol. Chem. 268(14):9960-9963 (1993).
Blackshear, P.J., J. Biol. Chem. 268(3):1501-1504 (1993).
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