|Application ||WB, E|
|Calculated MW||31555 Da|
|Antigen Region||241-271 aa|
|Other Names||Myristoylated alanine-rich C-kinase substrate, MARCKS, Protein kinase C substrate, 80 kDa protein, light chain, 80K-L protein, PKCSL, MARCKS, MACS, PRKCSL|
|Target/Specificity||This MARCKS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-271 amino acids from the C-terminal region of human MARCKS.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MARCKS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.|
|Cellular Location||Cytoplasm, cytoskeleton. Membrane; Lipid-anchor|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
MARCKS is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.
Rauch, M.E., et al., J. Biol. Chem. 277(16):14068-14076 (2002).
Aderem, A., Biochem. Soc. Trans. 23(3):587-591 (1995).
Rao, P.H., et al., Cytogenet. Cell Genet. 66(4):272-273 (1994).
Taniguchi, H., et al., J. Biol. Chem. 268(14):9960-9963 (1993).
Blackshear, P.J., J. Biol. Chem. 268(3):1501-1504 (1993).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.