|Application ||IHC-P, WB, E|
|Calculated MW||88368 Da|
|Antigen Region||606-636 aa|
|Other Names||Carnitine O-palmitoyltransferase 1, liver isoform, CPT1-L, Carnitine O-palmitoyltransferase I, liver isoform, CPT I, CPTI-L, Carnitine palmitoyltransferase 1A, CPT1A, CPT1|
|Target/Specificity||This CPT1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 606-636 amino acids from the C-terminal region of human CPT1A.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CPT1A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Plays an important role in triglyceride metabolism.|
|Cellular Location||Mitochondrion outer membrane; Multi- pass membrane protein|
|Tissue Location||Strong expression in kidney and heart, and lower in liver and skeletal muscle|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation.
Rasmussen, B.B., et al., J. Clin. Invest. 110(11):1687-1693 (2002).
Ogawa, E., et al., J. Hum. Genet. 47(7):342-347 (2002).
Cook, G.A., et al., Am. J. Med. Sci. 318(1):43-48 (1999).
IJlst, L., et al., J. Clin. Invest. 102(3):527-531 (1998).
Britton, C.H., et al., Genomics 40(1):209-211 (1997).
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