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>   home   >   Products   >   Primary Antibodies   >   MeCP2 Antibody (S421)   

MeCP2 Antibody (S421)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MeCP2 Antibody (S421) AP2545D
    Anti-MeCP2 Antibody (S421)at 1:2000 dilution + mouse brain lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - MeCP2 Antibody (S421) AP2545D
    Anti-MeCP2 Antibody (S421)at 1:2000 dilution + mouse brain lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - MeCP2 Antibody (S421) AP2545D
    Anti-MeCP2 Antibody (S421)at 1:1000 dilution + mouse brain lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - MeCP2 Antibody (S421) AP2545D
    All lanes : Anti-MeCP2 Antibody (S421) at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysates Lane 2: Jurkat whole cell lysates Lane 3: A431 whole cell lysates Lane 4: HepG2 whole cell lysates Lane 5: NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - MeCP2 Antibody (S421) AP2545D
    Anti-MeCP2 Antibody (S421)at 1:2000 dilution + mouse brain lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - MeCP2 Antibody (S421) AP2545D
    Western blot analysis of MeCP2-S421* (Cat.#AP2545d) in mouse brain, cerebellum tissue and HepG2 cell line lysates (35ug/lane). CP2 (arrow) was detected using the purified Pab.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q95LG8
Other Accession Q9Z2D6, P51608
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 52427 Da
Antigen Region 400-428 aa
Additional Information
Gene ID 102135563
Other Names Methyl-CpG-binding protein 2, MeCp-2 protein, MeCp2, MECP2
Target/Specificity This MeCP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-428 amino acids from human MeCP2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMeCP2 Antibody (S421) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MECP2
Function Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
Cellular Location Nucleus. Note=Colocalized with methyl-CpG in the genome.
Citations (0)

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Background

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

References

dos Santos, J.M., et al., Neurosci. Lett. 379(1):13-16 (2005).
Ylisaukko-Oja, T., et al., Am J Med Genet A 132(2):121-124 (2005).
Schanen, C., et al., Am J Med Genet A 126(2):129-140 (2004).
Shibayama, A., et al., Am. J. Med. Genet. B Neuropsychiatr. Genet. 128(1):50-53 (2004).
Fang, J.Y., et al., World J. Gastroenterol. 10(23):3394-3398 (2004).

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$ 295.00
$ 99.00
Cat# AP2545D
Size:
Quantity:
(40 western blots)
Availability: In Stock
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