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PHB Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • IHC-P - PHB Antibody (Center) AP2710C
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PHB antibody (Center) (Cat. #AP2710c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • WB - PHB Antibody (Center) AP2710C
    Western blot analysis of PHB Antibody (Center) (Cat.#AP2710c) in Hela cell line lysates (35ug/lane). PHB (arrow) was detected using the purified Pab.
  • WB - PHB Antibody (Center) AP2710C
    Western blot analysis of PHB (arrow) using rabbit polyclonal PHB Antibody (Center) (Cat.#AP2710c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PHB gene.
  • IF - PHB Antibody (Center) AP2710C
    Confocal immunofluorescent analysis of PHB Antibody (Center)(Cat#AP2710c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
  • FC - PHB Antibody (Center) AP2710C
    PHB Antibody (Center) (Cat. #AP2710c) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P35232
Other Accession P84173, Q3T165
Reactivity Human
Predicted Bovine, Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 29804 Da
Antigen Region 89-117 aa
Additional Information
Gene ID 5245
Other Names Prohibitin, PHB
Target/Specificity This PHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the Central region of human PHB.
Dilution IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPHB Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PHB
Function Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
Cellular Location Mitochondrion inner membrane.
Tissue Location Widely expressed in different tissues.
Research Areas
Citations (0)

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Prohibitin is an evolutionarily conserved protein that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region.


Gregory-Bass,R.C., Int. J. Cancer 122 (9), 1923-1930 (2008)
Ross,J.A., J. Biol. Chem. 283 (8), 4699-4713 (2008)
White,J.J., Genomics 11 (1), 228-230 (1991)

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Cat# AP2710C
(40 western blots)
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