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Deaf1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Deaf1 Antibody (C-term) AP2711b
    Western blot analysis of Deaf1(arrow) using rabbit polyclonal Deaf1 Antibody (Human C-term) (Cat.#AP2711b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Deaf1 gene (Lane 2) (Origene Technologies).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Z1T5
Other Accession O88450
Reactivity Human
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 59633 Da
Antigen Region 368-400 aa
Additional Information
Gene ID 54006
Other Names Deformed epidermal autoregulatory factor 1 homolog, Nuclear DEAF-1-related transcriptional regulator, NUDR, Deaf1
Target/Specificity This Deaf1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 368-400 amino acids from the C-terminal region of human Deaf1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDeaf1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Deaf1
Function Transcription factor that binds to sequence with multiple copies of 5'-TTC[CG]G-3' present in its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) 5'- AGGGTTCACCGAAAGTTCA-3'. Activates the proenkephalin gene independently of promoter binding, probably through protein- protein interaction (By similarity). Regulates epithelial cell proliferation and side-branching in the mammary gland. Required for neural tube closure and skeletal patterning. Controls the expression of peripheral tissue antigens in pancreatic lymph nodes. Isoform 1 displays greater transcriptional activity than isoform 2. Isoform 2 may inhibit transcriptional activity of isoform 1 by interacting with it and retaining it in the cytoplasm. Transcriptional activator of EIF4G3 (By similarity). May also involved in behavior (PubMed:24726472).
Cellular Location Isoform 1: Nucleus. Cytoplasm.
Tissue Location Ubiquitously expressed during embryogenesis, with higher expression in regions of the central nervous system, dorsal root ganglia, submandibular gland, epidermis and breast. In 12-week-old NOD mice, expression of isoform 2 is sevenfold higher in lymph node stromal elements than in T-cells and tenfold higher than in B-cells.
Research Areas
Citations (0)

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Drosophila Deformed epidermal autoregulatory factor 1 (DEAF-1) is a DNA-binding protein that interacts with regulatory sequences first described in the Deformed epidermal autoregulatory element.


Czesak,M.,J. Neurosci. 26 (6), 1864-1871 (2006)
Hahm,K., Mol. Cell. Biol. 24 (5), 2074-2082 (2004)

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$ 295.00
$ 99.00
Cat# AP2711b
(40 western blots)
Availability: In Stock
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