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NPTX2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NPTX2 Antibody (C-term) AP2718b
    Anti-NPTX2 (C-term) at 1:1000 dilution + H. pancreas whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - NPTX2 Antibody (C-term) AP2718b
    Formalin-fixed and paraffin-embedded human testis tissue reacted with NARP(Nptx2) Antibody (C-term) (Cat.#AP2718b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P47972
Other Accession P97738, O70340
Reactivity Human
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 309-338 aa
Additional Information
Other Names Neuronal pentraxin-2, NP2, Neuronal pentraxin II, NP-II, NPTX2
Target/Specificity This NPTX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-338 amino acids from the C-terminal region of human NPTX2.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNPTX2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NPTX2
Function Likely to play role in the modification of cellular properties that underlie long-term plasticity. Binds to agar matrix in a calcium-dependent manner (By similarity).
Cellular Location Secreted.
Tissue Location Brain, pancreas, liver, heart and skeletal muscle. Highest levels are seen in the testis EMBL; U29195; AAA68980.2; -; Genomic_DNA EMBL; U29191; AAA68980.2; JOINED; Genomic_DNA EMBL; U29192; AAA68980.2; JOINED; Genomic_DNA EMBL; U29193; AAA68980.2; JOINED; Genomic_DNA EMBL; U29194; AAA68980.2; JOINED; Genomic_DNA EMBL; U26662; AAA92296.1; -; mRNA EMBL; AC074121; AAQ93363.1; -; Genomic_DNA EMBL; CH236956; EAL23889.1; -; Genomic_DNA EMBL; CH471091; EAW76705.1; -; Genomic_DNA EMBL; BC009924; AAH09924.1; -; mRNA EMBL; BC034781; AAH34781.1; -; mRNA EMBL; BC035339; AAH35339.1; -; mRNA EMBL; BC048275; AAH48275.2; -; mRNA CCDS; CCDS5657.1; - RefSeq; NP_002514.1; NM_002523.2 UniGene; Hs.3281; - ProteinModelPortal; P47972; - SMR; P47972; - BioGrid; 110945; 6 IntAct; P47972; 1 STRING; 9606.ENSP00000265634; - iPTMnet; P47972; - PhosphoSitePlus; P47972; - BioMuta; NPTX2; - DMDM; 20981708; - EPD; P47972; - PaxDb; P47972; - PeptideAtlas; P47972; - PRIDE; P47972; - ProteomicsDB; 55826; - DNASU; 4885; - Ensembl; ENST00000265634; ENSP00000265634; ENSG00000106236 GeneID; 4885; - KEGG; hsa:4885; - UCSC; uc003upl.3; human CTD; 4885; - DisGeNET; 4885; - EuPathDB; HostDB:ENSG00000106236.3; - GeneCards; NPTX2; - HGNC; HGNC:7953; NPTX2 HPA; CAB020801; - HPA; HPA049799; - HPA; HPA058320; - MIM; 600750; gene neXtProt; NX_P47972; - OpenTargets; ENSG00000106236; - PharmGKB; PA31739; - eggNOG; ENOG410IFFF; Eukaryota eggNOG; ENOG410XR4W; LUCA GeneTree; ENSGT00760000119128; - HOGENOM; HOG000231267; - HOVERGEN; HBG003608; - InParanoid; P47972; - OMA; DNLAPWH; - OrthoDB; EOG091G0AHP; - PhylomeDB; P47972; - TreeFam; TF330208; - GeneWiki; NPTX2; - GenomeRNAi; 4885; - PRO; PR:P47972; - Proteomes; UP000005640; Chromosome 7 Bgee; ENSG00000106236; - CleanEx; HS_NPTX2; - Genevisible; P47972; HS GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW GO; GO:0008306; P:associative learning; IEA:Ensembl GO; GO:0007268; P:chemical synaptic transmission; NAS:UniProtKB CDD; cd00152; PTX; 1 InterPro; IPR013320; ConA-like_dom_sf InterPro; IPR030476; Pentaxin_CS InterPro; IPR001759; Pentraxin-related Pfam; PF00354; Pentaxin; 1 PRINTS; PR00895; PENTAXIN SMART; SM00159; PTX; 1 SUPFAM; SSF49899; SSF49899; 1 PROSITE; PS00289; PTX_1; 1 PROSITE; PS51828; PTX_2; 1 1: Evidence at protein level; Calcium; Complete proteome; Disulfide bond; Glycoprotein; Lectin; Metal-binding; Reference proteome; Secreted; Signal SIGNAL 1 15 CHAIN 16 431 Neuronal pentraxin-2 /FTId=PRO_0000023551 DOMAIN 223 424 Pentraxin (PTX). {ECO:0000255|PROSITE- ProRule:PRU01172} METAL 277 277 Calcium 1. METAL 355 355 Calcium 1. METAL 355 355 Calcium 2. {ECO:0000255|PROSITE- ProRule:PRU01172} METAL 356 356 Calcium 1; via carbonyl oxygen METAL 357 357 Calcium 1. METAL 357 357 Calcium 2. {ECO:0000255|PROSITE- ProRule:PRU01172} METAL 367 367 Calcium 2. {ECO:0000255|PROSITE- ProRule:PRU01172} CARBOHYD 148 148 N-linked (GlcNAc...) asparagine CARBOHYD 189 189 N-linked (GlcNAc...) asparagine CARBOHYD 393 393 N-linked (GlcNAc...) asparagine DISULFID 253 313 {ECO:0000255|PROSITE-ProRule:PRU01172} CONFLICT 78 82 GAQRE -> ASAR (in Ref. 1; AAA68980/ AAA92296). CONFLICT 280 280 V -> L (in Ref. 1; AAA68980/AAA92296) CONFLICT 427 427 R -> A (in Ref. 1; AAA68980/AAA92296) SEQUENCE 431 AA; 47042 MW; 87B7BF9BA1049AC5 CRC64; MLALLAASVA LAVAAGAQDS PAPGSRFVCT ALPPEAVHAG CPLPAMPMQG GAQSPEEELR AAVLQLRETV VQQKETLGAQ REAIRELTGK LARCEGLAGG KARGAGATGK DTMGDLPRDP GHVVEQLSRS LQTLKDRLES LEHQLRANVS NAGLPGDFRE VLQQRLGELE RQLLRKVAEL EDEKSLLHNE TSAHRQKTES TLNALLQRVT ELERGNSAFK SPDAFKVSLP LRTNYLYGKI KKTLPELYAF TICLWLRSSA SPGIGTPFSY AVPGQANEIV LIEWGNNPIE LLINDKVAQL PLFVSDGKWH HICVTWTTRD GMWEAFQDGE KLGTGENLAP WHPIKPGGVL ILGQEQDTVG GRFDATQAFV GELSQFNIWD RVLRAQEIVN IANCSTNMPG NIIPWVDNNV DVFGGASKWP VETCEERLLD L
Research Areas
Citations (0)

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Background

NPTX2 is likely to play a role in the modification of cellular properties that underlie long-term plasticity. It binds to agar matrix in a calcium-dependent manner.

References

Park,J.K., Pancreas 35 (3), E9-E15 (2007)
Marui,T., Prog. Neuropsychopharmacol. Biol. Psychiatry 31 (4), 940-943 (2007)
Kirkpatrick,L.L., J. Biol. Chem. 275 (23), 17786-17792 (2000)
Dodds,D.C., . Biol. Chem. 272 (34), 21488-21494 (1997)
Hsu,Y.C., Genomics 28 (2), 220-227 (1995)

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$ 295.00
$ 99.00
Cat# AP2718b
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(40 western blots)
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