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PHPT1 Antibody( C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PHPT1 Antibody( C-term) AP2721b
    Western blot analysis of PHPT1 (arrow) using rabbit polyclonal PHPT1 Antibody (Human C-term) (Cat#.AP2127b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHPT1 gene (Lane 2) (Origene Technologies).
    detail
  • IHC-P - PHPT1 Antibody( C-term) AP2721b
    Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PHPT1 Antibody (N-term) (Cat.#AP2721b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9NRX4
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 13833 Da
Antigen Region 88-117 aa
Additional Information
Gene ID 29085
Other Names 14 kDa phosphohistidine phosphatase, 313-, Phosphohistidine phosphatase 1, Protein janus-A homolog, PHPT1, PHP14
Target/Specificity This PHPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the C-terminal region of human PHPT1.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPHPT1 Antibody( C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PHPT1
Synonyms PHP14
Function Exhibits phosphohistidine phosphatase activity.
Cellular Location Cytoplasm.
Tissue Location Expressed abundantly in heart and skeletal muscle.
Research Areas
Citations (0)
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Background

PHPT1 is an EDTA-insensitive phosphohistidine phosphatase that catalyzes the dephosphorylation of phosphopeptide I (Ek etal., 2002 [PubMed 12383260]).[supplied by OMIM].

References

Lim,J., Cell 125 (4), 801-814 (2006)
Mehrle,A., Nucleic Acids Res. 34 (DATABASE ISSUE), D415-D418 (2006)
Ma,R.,Biochem. Biophys. Res. Commun. 337 (3), 887-891 (2005)

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$ 365.00
$ 140.00
Cat# AP2721b
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