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AKR1B1 Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP2735b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP2735b-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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- Citiations : 0
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- Backgrounds
AKR1B1 Antibody (C-term) - Product info | |
| Application | IHC, WB, IF
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| Primary Accession | P15121 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 35853 Da |
AKR1B1 Antibody (C-term) - Additional info | |
| Gene ID 231 | |
| Other Names AKR1B1; ALDR1; Aldose reductase; Aldehyde reductase; Aldo-keto reductase family 1 member B1 | |
| Target/Specificity This AKR1B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287~316 amino acids from the C-terminal region of human AKR1B1. | |
| Dilution IHC~~1:10~50 WB~~1:50~100WB~~1:1000 IF~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions AKR1B1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
AKR1B1 Antibody (C-term) - Protein Information | |
| Name AKR1B1 | |
| Synonyms ALDR1 | |
| Function Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies | |
| Cellular Location Cytoplasm. | |
| Tissue Location Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress | |
AKR1B1 Antibody (C-term) - Related products
AP2735b: AKR1B1 Antibody (C-term)
AP2735c: AKR1B1 Antibody (Center)
RI10208: AKR1B1 predesign siRNA
AKR1B1 Antibody (C-term) - Application data
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Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AKR1B1 antibody (C-term) (Cat.#AP2735b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Western blot analysis of anti-AKR1B1 Pab (Cat.#AP2735b) in A375 cell line lysates (35ug/lane).AKR1B1 (arrow) was detected using the purified Pab (1:60 dilution).
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Western blot analysis of AKR1B1 (arrow) using rabbit polyclonal AKR1B1 Antibody (C-term) (Cat.#AP2735b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AKR1B1 gene.
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Confocal immunofluorescent analysis of AKR1B1 Antibody (C-term)(Cat#AP2735b) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
AKR1B1 Antibody (C-term) - Research Areas
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BACKGROUND
AKR1B1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This protein catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol.
REFERENCES
Steuber,H.,J. Mol. Biol. 379 (5), 991-1016 (2008) Gleissner,C.A.,Arterioscler. Thromb. Vasc. Biol. 28 (6), 1137-1143 (2008) Grundmann,U.,DNA Cell Biol. 9 (3), 149-157 (1990)
