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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Vimentin Antibody (C-term)   

Vimentin Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Vimentin Antibody (C-term) AP2739B
    Anti-Vimentin Antibody (C-term) at 1:1000 dilution + U-2OS whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IF - Vimentin Antibody (C-term) AP2739B
    Fluorescent confocal image of SY5Y cells stained with Vimentin (C-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP2739b Vimentin (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the Vimentin immunosignal to the cytoskeleton, supported by Human Protein Atlas Data (
  • WB - Vimentin Antibody (C-term) AP2739B
    Western blot analysis of Vimentin antibody (C-term) (Cat.#AP2739b) in NCI-H460 cell line lysates (35ug/lane). Vimentin (arrow) was detected using the purified Pab .
  • IHC-P - Vimentin Antibody (C-term) AP2739B
    Vimentin Antibody (C-term) (Cat. #AP2739b)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Vimentin Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - Vimentin Antibody (C-term) AP2739B
    Vimentin Antibody (C-term) (Cat. #AP2739b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08670
Other Accession P31000, P02543, P20152, Q4R4X4, P48670, P09654, P48616, P24790
Reactivity Human
Predicted Xenopus, Bovine, Chicken, Hamster, Monkey, Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 53652 Da
Antigen Region 430-457 aa
Additional Information
Gene ID 7431
Other Names Vimentin, VIM
Target/Specificity This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 430-457 amino acids from the C-terminal region of human Vimentin.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVimentin Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VIM
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Cellular Location Cytoplasm.
Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines
Research Areas
Citations ( 0 )


Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.


References for protein:
1.Whipple,R.A.,Cancer Res. 68 (14), 5678-5688 (2008)
2.Garcia-Verdugo,I.,Biochemistry 47 (18), 5127-5138 (2008)
3.Merdes,A., J. Cell Biol. 115 (2), 397-410 (1991)
References for SY5Y (SH-SY5Y; ATCC#CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704]

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$ 295.00
$ 99.00
Cat# AP2739B
(40 western blots)
Availability: In Stock
Bulk Size
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