|Application ||WB, IHC-P, FC, E|
|Other Accession||P31000, P02543, P20152, P48670, P48616|
|Predicted||Bovine, Hamster, Mouse, Pig, Rat|
|Calculated MW||53652 Da|
|Antigen Region||152-181 aa|
|Other Names||Vimentin, VIM|
|Target/Specificity||This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-181 amino acids from the Central region of human Vimentin.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Vimentin Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.|
|Tissue Location||Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines|
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Provided below are standard protocols that you may find useful for product applications.
Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.
Whipple,R.A.,Cancer Res. 68 (14), 5678-5688 (2008)
Garcia-Verdugo,I.,Biochemistry 47 (18), 5127-5138 (2008)
Merdes,A., J. Cell Biol. 115 (2), 397-410 (1991)
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