- Antibodies
- New
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Tag
- Peptides
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Amino Acids
- Tag
- Biological Process >
- Proteins
- RNAi
- FL cDNA Clones
- Cell/Tissues/Lysates
Vimentin Antibody (Center)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
Australia Austria Belgium Brazil Bulgaria Canada China Croatia Cyprus Czech Republic Denmark Estonia Finland France Germany Greece Hungary Iceland India Indonesia Ireland Israel Italy Japan Korea Latvia Lithuania Luxembourg Macedonia Malaysia Malta Netherlands Norway Pakistan Poland Portugal Romania Serbia Singapore Slovakia Slovenia Spain Sweden Switzerland Taiwan Turkey United Kingdom United States Vietnam Others 
|
|||
|---|---|---|---|---|
| Catalog # | Size | Availability | Price | |
| AP2739c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
Vimentin Antibody (Center) - Product info | |
| Application | WB, IHC, FC
|
| Primary Accession | P08670 |
| Other Accession | P31000, P02543, P20152, P48670, P48616 |
| Reactivity | Human |
| Predicted | Bovine, Hamster, Mouse, Pig, Rat |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 53652 Da |
Vimentin Antibody (Center) - Additional info | |
| Gene ID 7431 | |
| Other Names VIM; Vimentin | |
| Target/Specificity This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159~189 amino acids from the center region of human Vimentin. | |
| Dilution WB~~1:50~100WB~~1:1000 IHC~~1:10~50 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Vimentin Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
Vimentin Antibody (Center) - Protein Information | |
| Name VIM | |
| Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally | |
| Cellular Location Cytoplasm. | |
| Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines | |
Vimentin Antibody (Center) - Related products
AP2739a: Vimentin Antibody (S82)
AP2739b: Vimentin Antibody (C-term)
AP2739c: Vimentin Antibody (Center)
AP3546a: Phospho-Vimentin-S82 Antibody
LY10969a: VIM Over-expression Lysate
BP2739a: Vimentin Antibody (S82) Blocking Peptide
BP2739b: Vimentin Antibody (C-term) Blocking Peptide
BP2739c: Vimentin Antibody (Center) Blocking Peptide
BP3546a: Phospho-Vimentin-S82 Antibody Blocking Peptide
AT4513a: VIM Antibody (monoclonal) (M01)
AJ1815c: Vimentin Antibody Phospho (pS38)
Vimentin Antibody (Center) - Application data
-
Western blot analysis of Vimentin Antibody (Center) (Cat. #AP2739c) in A2058,A375 cell line lysates (35ug/lane). Vimentin (arrow) was detected using the purified Pab.
-
Western blot analysis of Vimentin (arrow) using Vimentin Antibody (Center) (Cat. #AP2739c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (Lane 2) (Origene Technologies).
-
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
-
Vimentin Antibody (Center) (Cat. #AP2739c)immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Vimentin Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
-
Vimentin Antibody (Center) (Cat. #AP2739c) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Vimentin Antibody (Center) - Research Areas
Abgent welcomes feedback from its customers.
If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Thank you for your support.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.
REFERENCES
Whipple,R.A.,Cancer Res. 68 (14), 5678-5688 (2008) Garcia-Verdugo,I.,Biochemistry 47 (18), 5127-5138 (2008) Merdes,A., J. Cell Biol. 115 (2), 397-410 (1991)
