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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Vimentin Antibody (Center)   

Vimentin Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Vimentin Antibody (Center) AP2739c
    Western blot analysis of Vimentin Antibody (Center) (Cat. #AP2739c) in A2058,A375 cell line lysates (35ug/lane). Vimentin (arrow) was detected using the purified Pab.
  • WB - Vimentin Antibody (Center) AP2739c
    Western blot analysis of Vimentin (arrow) using Vimentin Antibody (Center) (Cat. #AP2739c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (Lane 2) (Origene Technologies).
  • IHC-P - Vimentin Antibody (Center) AP2739c
    Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IHC-P - Vimentin Antibody (Center) AP2739c
    Vimentin Antibody (Center) (Cat. #AP2739c)immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Vimentin Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - Vimentin Antibody (Center) AP2739c
    Vimentin Antibody (Center) (Cat. #AP2739c) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08670
Other Accession P31000, P02543, P20152, P48670, P48616
Reactivity Human
Predicted Bovine, Hamster, Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 53652 Da
Antigen Region 152-181 aa
Additional Information
Gene ID 7431
Other Names Vimentin, VIM
Target/Specificity This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-181 amino acids from the Central region of human Vimentin.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVimentin Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VIM
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Cellular Location Cytoplasm.
Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines
Research Areas
Citations (0)

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Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.


Whipple,R.A.,Cancer Res. 68 (14), 5678-5688 (2008)
Garcia-Verdugo,I.,Biochemistry 47 (18), 5127-5138 (2008)
Merdes,A., J. Cell Biol. 115 (2), 397-410 (1991)

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Cat# AP2739c
(40 western blots)
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