|Application ||IHC-P, WB, E|
|Calculated MW||99998 Da|
|Antigen Region||605-638 aa|
|Other Names||Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, LGR5, GPR49, GPR67|
|Target/Specificity||This LGR5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 605-638 amino acids from human LGR5.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||LGR5 (GPR49) Antibody (loop1) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R- spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.|
|Cellular Location||Cell membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Note=Rapidly and constitutively internalized to the trans-Golgi network at steady state. Internalization to the trans- Golgi network may be the result of phosphorylation at Ser-861 and Ser-864; however, the phosphorylation event has not been proven (PubMed:23439653).|
|Tissue Location||Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver|
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Provided below are standard protocols that you may find useful for product applications.
LGR5/GPR49 is an orphan receptor. It may be an important receptor for signals controlling growth and differentiation of specific embryonic tissues.
Barker,N., Nature 449 (7165), 1003-1007 (2007) McClanahan,T., Cancer Biol. Ther. 5 (4), 419-426 (2006) Yamamoto,Y., Hepatology 37 (3), 528-533 (2003) Hsu,S.Y., Mol. Endocrinol. 14 (8), 1257-1271 (2000)
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