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LGR5 (GPR49) Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - LGR5 (GPR49) Antibody (Center) AP2745f
    Western blot analysis of anti-LGR5/GPR49 Antibody (Center) (Cat.#AP2745f) in K562 cell line lysates (35ug/lane). LGR5/GPR49(arrow) was detected using the purified Pab.
  • IHC-P - LGR5 (GPR49) Antibody (Center) AP2745f
    Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody (Center) (Cat.#AP2745f), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • WB - LGR5 (GPR49) Antibody (Center) AP2745f
    LGR5/GPR49 Antibody (Center) (Cat.#AP2745f) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow)(Kindly offered by Dr. Li).
  • FC - LGR5 (GPR49) Antibody (Center) AP2745f
    LGR5 (GPR49) Antibody (Center) (Cat. #AP2745f) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75473
Other Accession F1MT22
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 99998 Da
Antigen Region 443-473 aa
Additional Information
Gene ID 8549
Other Names Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, LGR5, GPR49, GPR67
Target/Specificity This LGR5 (GPR49) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 443-473 amino acids from the Central region of human LGR5 (GPR49).
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLGR5 (GPR49) Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LGR5
Synonyms GPR49, GPR67
Function Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R- spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
Cellular Location Cell membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Note=Rapidly and constitutively internalized to the trans-Golgi network at steady state. Internalization to the trans- Golgi network may be the result of phosphorylation at Ser-861 and Ser-864; however, the phosphorylation event has not been proven (PubMed:23439653).
Tissue Location Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver
Research Areas
Citations ( 0 )


LGR5/GPR49 is an orphan receptor. It may be an important receptor for signals controlling growth and differentiation of specific embryonic tissues.


Barker,N., Nature 449 (7165), 1003-1007 (2007) McClanahan,T., Cancer Biol. Ther. 5 (4), 419-426 (2006) Yamamoto,Y., Hepatology 37 (3), 528-533 (2003) Hsu,S.Y., Mol. Endocrinol. 14 (8), 1257-1271 (2000)

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$ 295.00
$ 99.00
Cat# AP2745f
(40 western blots)
Availability: In Stock
Bulk Size
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