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MGST1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MGST1 Antibody (Center) AP2750c
    Anti-MGST1 Antibody (Center) at 1:4000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 18 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P10620
Other Accession Q64L89
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 17599 Da
Antigen Region 40-71 aa
Additional Information
Gene ID 4257
Other Names Microsomal glutathione S-transferase 1, Microsomal GST-1, Microsomal GST-I, MGST1, GST12, MGST
Target/Specificity This MGST1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-71 amino acids from the Central region of human MGST1.
Dilution WB~~1:4000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMGST1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MGST1
Synonyms GST12, MGST
Function Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Cellular Location Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:P08011}; Multi-pass membrane protein. Mitochondrion outer membrane {ECO:0000250|UniProtKB:P08011}
Tissue Location Highly expressed in liver.
Research Areas
Citations (0)
citation

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Background

The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds.This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress.

References

Zhang,H., Int J Colorectal Dis 22 (10), 1185-1194 (2007)
Prall,W.C., Mech. Ageing Dev. 128 (9), 503-510 (2007)
Haines,J.L.,Invest. Ophthalmol. Vis. Sci. 47 (1), 329-335 (2006)

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$ 182.50
$ 70.00
Cat# AP2750c
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