|Application ||WB, E|
|Calculated MW||50246 Da|
|Antigen Region||337-366 aa|
|Other Names||Telomeric repeat-binding factor 1, NIMA-interacting protein 2, TTAGGG repeat-binding factor 1, Telomeric protein Pin2/TRF1, TERF1, PIN2, TRBF1, TRF, TRF1|
|Target/Specificity||This TRF (TERF1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 337-366 amino acids from the C-terminal region of human TRF (TERF1).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TRF (TERF1) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||PIN2, TRBF1, TRF, TRF1|
|Function||Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double- stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.|
|Cellular Location||Nucleus. Cytoplasm, cytoskeleton, spindle. Chromosome, telomere. Note=Colocalizes with telomeric DNA in interphase and prophase cells. Telomeric localization decreases in metaphase, anaphase and telophase. Associates with the mitotic spindle|
|Tissue Location||Highly expressed and ubiquitous. Isoform Pin2 predominates|
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Provided below are standard protocols that you may find useful for product applications.
TERF1 is a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus.
Kim,M.K., J. Biol. Chem. 283 (20), 14144-14152 (2008)
Etheridge,K.T., J. Biol. Chem. 283 (11), 6935-6941 (2008)
Muramatsu,Y., Exp. Cell Res. 314 (5), 1115-1124 (2008)
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