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UGP2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - UGP2 Antibody (C-term) AP2760b
    All lanes : Anti-UGP2 Antibody (C-term) at 1:2000 dilution Lane 1: A2058 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - UGP2 Antibody (C-term) AP2760b
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with UGP2 antibody (C-term) (Cat.#AP2760b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
  • CITATIONS: 2
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q16851
Other Accession P79303, Q91ZJ5, O35156, Q07130
Reactivity Human
Predicted Bovine, Hamster, Mouse, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 56940 Da
Antigen Region 467-497 aa
Additional Information
Gene ID 7360
Other Names UTP--glucose-1-phosphate uridylyltransferase, UDP-glucose pyrophosphorylase, UDPGP, UGPase, UGP2, UGP1
Target/Specificity This UGP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 467-497 amino acids from the C-terminal region of human UGP2.
Dilution WB~~1:2000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUGP2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UGP2 (HGNC:12527)
Function UTP--glucose-1-phosphate uridylyltransferase catalyzing the conversion of glucose-1-phosphate into UDP-glucose, a crucial precursor for the production of glycogen.
Cellular Location Cytoplasm
Tissue Location Highly expressed in various brain regions. Expressed in amygdala, anterior cingulate cortex, caudate, cerebellar hemisphere, cerebellum, cortex, frontal cortex, hippocampus, hypothalamus, nucleus accumbens, putamen, spinal cord and substantia nigra (PubMed:31820119). Also widely expressed among other tissues, including liver, heart, placenta, lung, kidney, pancreas and skeletal muscle (PubMed:8354390, PubMed:8631325).
Research Areas
Citations ( 0 )

Background

UGP2 is an important intermediary in mammalian carbohydrate interconversions. It transfers a glucose moiety from glucose-1-phosphate to MgUTP and forms UDP-glucose and MgPPi. In liver and muscle tissue,UDP-glucose is a direct precursor of glycogen; in lactating mammary gland it is converted to UDP-galactose which is then converted to lactose. The eukaryotic enzyme has no significant sequence similarity to the prokaryotic enzyme.

References

Ewing,R.M., Mol. Syst. Biol. 3, 89 (2007) Wistow,G., (er) Mol. Vis. 8, 205-220 (2002) Chang,H.Y., Eur. J. Biochem. 236 (2), 723-728 (1996)

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$ 182.50
$ 70.00
Cat# AP2760b
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