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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   MCAM Antibody (C-term)   

MCAM Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MCAM Antibody (C-term) AP2767b
    Western blot analysis of MCAM Antibody (C-term) (Cat. #AP2767b) in A2058 cell line lysates (35ug/lane). MCAM (arrow) was detected using the purified Pab.
  • IHC-P - MCAM Antibody (C-term) AP2767b
    MCAM Antibody (C-term) (Cat. #AP2767b)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MCAM Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P43121
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 71607 Da
Antigen Region 532-558 aa
Additional Information
Gene ID 4162
Other Names Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen, CD146, MCAM, MUC18
Target/Specificity This MCAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 532-558 amino acids from the C-terminal region of human MCAM.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMCAM Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MCAM
Synonyms MUC18
Function Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis
Research Areas
Citations (0)

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Background

MCAM plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. It could be an adhesion molecule active in neural crest cells during embryonic development. It acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.

References

Fritzsche,F.R., Pathology 40 (5), 457-464 (2008)
Malyszko,J., Clin. Appl. Thromb. Hemost. 14 (3), 338-345 (2008)
Guezguez,B., J. Immunol. 179 (10), 6673-6685 (2007)

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$ 295.00
$ 99.00
Cat# AP2767b
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Quantity:
(40 western blots)
Availability: In Stock
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