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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   FBL Antibody (Center E120)   

FBL Antibody (Center E120)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - FBL Antibody (Center E120) AP2776c
    Western blot analysis of anti-FBL Antibody (Center E120) (Cat.#AP2776c) in K562 cell line lysates (35ug/lane). FBL (arrow) was detected using the purified Pab.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P22087
Other Accession P22509, P35550
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 33784 Da
Antigen Region 105-135 aa
Additional Information
Gene ID 2091
Other Names rRNA 2'-O-methyltransferase fibrillarin, 211-, 34 kDa nucleolar scleroderma antigen, Histone-glutamine methyltransferase, FBL, FIB1, FLRN
Target/Specificity This FBL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-135 amino acids from the Central region of human FBL.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFBL Antibody (Center E120) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FBL
Synonyms FIB1, FLRN
Function S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).
Cellular Location Nucleus, nucleolus Note=Fibrillar region of the nucleolus
Research Areas
Citations (0)

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FBL is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. This protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.


Amin,M.A., Biochem. Biophys. Res. Commun. 360 (2), 320-326 (2007)
Dunphy,J.L., Traffic 8 (6), 661-672 (2007)

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$ 295.00
$ 99.00
Cat# AP2776c
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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