|Application ||WB, E|
|Other Accession||P22509, P35550|
|Calculated MW||33784 Da|
|Antigen Region||105-135 aa|
|Other Names||rRNA 2'-O-methyltransferase fibrillarin, 211-, 34 kDa nucleolar scleroderma antigen, Histone-glutamine methyltransferase, FBL, FIB1, FLRN|
|Target/Specificity||This FBL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-135 amino acids from the Central region of human FBL.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||FBL Antibody (Center E120) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).|
|Cellular Location||Nucleus, nucleolus Note=Fibrillar region of the nucleolus|
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Provided below are standard protocols that you may find useful for product applications.
FBL is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. This protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.
Amin,M.A., Biochem. Biophys. Res. Commun. 360 (2), 320-326 (2007)
Dunphy,J.L., Traffic 8 (6), 661-672 (2007)
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