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GPC4 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GPC4 Antibody (Center) AP2790c
    Anti-GPC4 Antibody (Center) at 1:500 dilution + U-2OS whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 62 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O75487
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 62412 Da
Antigen Region 395-422 aa
Additional Information
Gene ID 2239
Other Names Glypican-4, K-glypican, Secreted glypican-4, GPC4
Target/Specificity This GPC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395-422 amino acids from the Central region of human GPC4.
Dilution WB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGPC4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GPC4
Function Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system (By similarity).
Cellular Location Cell membrane; Lipid-anchor, GPI- anchor; Extracellular side
Research Areas
Citations (0)
citation

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Background

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome.

References

Karumanchi,S.A., Mol. Cell 7 (4), 811-822 (2001)
Hagihara,K., Dev. Dyn. 219 (3), 353-367 (2000)
Veugelers,M., Hum. Mol. Genet. 9 (9), 1321-1328 (2000)
Veugelers,M., Genomics 53 (1), 1-11 (1998)

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$ 365.00
$ 140.00
Cat# AP2790c
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